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Literature summary extracted from

  • Iijima, Y.; Wang, G.; Fridman, E.; Pichersky, E.
    Analysis of the enzymatic formation of citral in the glands of sweet basil (2006), Arch. Biochem. Biophys., 448, 141-149.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.183 GEDH1, phylogenetic tree and analysis, sequence comparison, expression in Escherichia coli Ocimum basilicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.183 Zn2+ 66% inhibition at 5 mM Ocimum basilicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.183 0.03
-
 geraniol pH 9.5 Ocimum basilicum
1.1.1.183 0.037
-
 nerol pH 9.5 Ocimum basilicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.183 additional information the enzyme is not affected by Na+, K+, NH4+, Ca2+, and Mg2+ Ocimum basilicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.183 geraniol + NADP+ Ocimum basilicum the back reaction is catalyzed by CAD1, the cinnamyl alcohol dehydrogenase in vivo, the geraniol,/nerol pathway involves sveral dehydrogenases, overview geranial + NADPH
-
 ir
1.1.1.183 nerol + NADP+ Ocimum basilicum
-
 neral + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.183 Ocimum basilicum Q2KNL6 GEDH1; cv. Sweet Dani, gene GEDH1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.183 recombinant GEDH1 from Escherichia coli by anion exchange chromatography Ocimum basilicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.183 leaf highest activity in young leaves decreasing to 60% in older leaves Ocimum basilicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.183 3-methyl-2-buten-1-ol + NADP+ 22.7% of the activity with geraniol Ocimum basilicum 3-methyl-2-butenal + NADPH
-
 ?
1.1.1.183 3-methyl-3-buten-1-ol + NADP+ 6.5% of the activity with geraniol Ocimum basilicum 3-methyl-3-butenal + NADPH
-
 ?
1.1.1.183 benzylalcohol + NADP+ 5.5% of the activity with geraniol Ocimum basilicum benzaldehyde + NADPH
-
 ?
1.1.1.183 cinnamyl alcohol + NADP+ 53.0% of the activity with geraniol Ocimum basilicum cinnamyl aldehyde + NADPH
-
 ?
1.1.1.183 cis-3-hexenol + NADP+ 13.3% of the activity with geraniol Ocimum basilicum cis-3-hexenal + NADPH
-
 ?
1.1.1.183 citronellol + NADP+ 16.6% of the activity with geraniol Ocimum basilicum citronellal + NADPH
-
 r
1.1.1.183 geraniol + NADP+ the back reaction is catalyzed by CAD1, the cinnamyl alcohol dehydrogenase in vivo, the geraniol,/nerol pathway involves sveral dehydrogenases, overview Ocimum basilicum geranial + NADPH
-
 ir
1.1.1.183 geraniol + NADP+ the back reaction is catalyzed by CAD1, the cinnamyl alcohol dehydrogenase in vivo Ocimum basilicum geranial + NADPH
-
 ?
1.1.1.183 hexanol + NADP+ 31.6% of the activity with geraniol Ocimum basilicum hexanal + NADPH
-
 ?
1.1.1.183 additional information no activity with coniferyl alcohol, menthol, carveol, dihydrocarveol, 3,7-dimethyl-octanol, ethanol, butanol, and isobutanol Ocimum basilicum ?
-
 ?
1.1.1.183 nerol + NADP+
-
 Ocimum basilicum neral + NADPH + H+
-
 r
1.1.1.183 nerol + NADP+ 101% of the activity with geraniol Ocimum basilicum neral + NADPH + H+
-
 r
1.1.1.183 phenylethanol + NADP+ 10.2% of the activity with geraniol Ocimum basilicum phenylethanal + NADPH
-
 ?
1.1.1.183 phenylpropanol + NADP+ 22.1% of the activity with geraniol Ocimum basilicum phenylpropanal + NADPH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.183 GEDH1
-
 Ocimum basilicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.183 9.5
-
-
 Ocimum basilicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.183 8.5 10 over 72% of maximal activity at pH 8.5 and pH 10.0 Ocimum basilicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.183 NADP+
-
 Ocimum basilicum
1.1.1.183 NADPH
-
 Ocimum basilicum