Literature summary extracted from

Gutierrez-Gonzalez, L.H.; Rojo-Dominguez, A.; Cabrera-Gonzalez, N.E.; Perez-Montfort, R.; Padilla-Zuniga, A.J.
Loosely packed papain prosegment displays inhibitory activity (2006), Arch. Biochem. Biophys., 446, 151-160.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
3.4.22.2	additional information	loosely packed papain prosegment displays inhibitory activity but can also function as activator for the mature enzyme, overview	Carica papaya

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.4.22.2	DNA sequence determination and analysis of the proenzyme, expression of the isolated papain prosegment in Escherichia coli strain BL21(DE3) in the insoluble fraction	Carica papaya

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.4.22.2	additional information	loosely packed papain prosegment displays inhibitory activity but can also function as activator for the mature enzyme, overview	Carica papaya

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.4.22.2	additional information	-	additional information	thermodynamics of the thermal unfolding of the recombinant prosegment, overview, pH-jump kinetics of the prosegment, overview	Carica papaya

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.22.2	Carica papaya	P00784	enzyme precursor	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
3.4.22.2	proteolytic modification	the proenzyme is activated by cleavage of the prosegment	Carica papaya

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.4.22.2	recombinant papain prosegment solubilized and refolded from Escherichia coli strain BL21(DE3) inclusion bodies, to homogeneity	Carica papaya

Renatured (Commentary)

EC Number	Renatured (Comment)	Organism
3.4.22.2	recombinant papain prosegment from Escherichia coli strain BL21(DE3) inclusion bodies by 2% Triton X-100, 8 M guanidinium hydrochloride, and dialysis against 50 mM phosphoric acid/NaOH buffer, pH 7.0, and centrifugation at 45000 rpm for 1 h	Carica papaya

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.22.2	additional information	molecular recognition of mature enzyme and prosegment, overview	Carica papaya	?	-	?
3.4.22.2	Nalpha-benzoyl-DL-Arg-4-nitroanilide + H2O	-	Carica papaya	Nalpha-benzoyl-DL-Arg + 4-nitroaniline	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.4.22.2	More	the papain prosegment shows a high degree of conformational flexibility, proenzyme structure molecular modeling	Carica papaya

Synonyms

EC Number	Synonyms	Comment	Organism
3.4.22.2	papaya proteinase 1	-	Carica papaya
3.4.22.2	PPI	-	Carica papaya

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
3.4.22.2	20	-	assay at	Carica papaya

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
3.4.22.2	additional information	-	thermal unfolding of the prosegment, in acid medium the enzyme unfolds in a globule-like conformation, irreversible without intermediate states, overview	Carica papaya

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.4.22.2	additional information	-	pH-jump kinetics of the prosegment at pH 8 to 3.4	Carica papaya
3.4.22.2	6.6	-	assay at	Carica papaya

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
3.4.22.2	0.0000019	-	papain prosegment	recombinant prosegment, pH 7.0, 20°C, inhibition kinetics	Carica papaya

pI Value

EC Number	Organism	Comment	pI Value Maximum	pI Value
3.4.22.2	Carica papaya	recombinnat prosegment, isoelectric focusing	-	4.4

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Release 2023.1 (January 2023)