EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.99.6 | K208E | entirely inactive in absence of allosteric activator N-acetylglucose-6-phosphate | Escherichia coli |
3.5.99.6 | K208V | homotropic cooperativity, Hill-coefficient 1.7, 2fold increase in dissociation constant for allosteric activator N-acetylglucose-6-phosphate compared to wild-type | Escherichia coli |
3.5.99.6 | R172A | inactive in absence of allosteric activator N-acetylglucose-6-phosphate, at high activator levels, cooperativity diminishes and substrate inhibition becomes significant | Escherichia coli |
3.5.99.6 | R172A/K208E | inactive in absence of allosteric activator N-acetylglucose-6-phosphate, at high activator levels, cooperativity diminishes and substrate inhibition becomes significant | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.99.6 | 0.33 | - |
D-glucosamine 6-phosphate | mutant R172A/K208E, Michaelis-Hill equation | Escherichia coli | |
3.5.99.6 | 0.55 | - |
D-glucosamine 6-phosphate | wild-type, Michaelis-Hill equation | Escherichia coli | |
3.5.99.6 | 0.61 | - |
D-glucosamine 6-phosphate | mutant K208E, Michaelis-Hill equation | Escherichia coli | |
3.5.99.6 | 0.69 | - |
D-glucosamine 6-phosphate | mutant K208V, Michaelis-Hill equation | Escherichia coli | |
3.5.99.6 | 1.67 | - |
D-glucosamine 6-phosphate | mutant R172A, Michaelis-Hill equation | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.99.6 | Escherichia coli | P0A759 | - |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.5.99.6 | alpha-D-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3 | residue R172 has a specific role in binding the substrate to the enzyme in the T state | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.99.6 | D-glucosamine 6-phosphate + H2O | - |
Escherichia coli | D-fructose 6-phosphate + NH3 | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.99.6 | 0.044 | - |
D-glucosamine 6-phosphate | mutant K208E, Michaelis-Hill equation, presence of saturating concentration of allosteric activator N-acetylglucose-6-phosphate | Escherichia coli | |
3.5.99.6 | 6.1 | - |
D-glucosamine 6-phosphate | mutant R172A/K208E, Michaelis-Hill equation, presence of saturating concentration of allosteric activator N-acetylglucose-6-phosphate | Escherichia coli | |
3.5.99.6 | 7.8 | - |
D-glucosamine 6-phosphate | mutant K208V, Michaelis-Hill equation, presence of saturating concentration of allosteric activator N-acetylglucose-6-phosphate | Escherichia coli | |
3.5.99.6 | 11.8 | - |
D-glucosamine 6-phosphate | mutant R172A, Michaelis-Hill equation, presence of saturating concentration of allosteric activator N-acetylglucose-6-phosphate | Escherichia coli | |
3.5.99.6 | 160 | - |
D-glucosamine 6-phosphate | wild-type, Michaelis-Hill equation, presence of saturating concentration of allosteric activator N-acetylglucose-6-phosphate | Escherichia coli |