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Literature summary extracted from

  • Record, E.; Asther, M.; Sigoillot, C.; Pages, S.; Punt, P.J.; Delattre, M.; Haon, M.; van den Hondel, C.A.; Sigoillot, J.C.; Lesage-Meessen, L.
    Overproduction of the Aspergillus niger feruloyl esterase for pulp bleaching application (2003), Appl. Microbiol. Biotechnol., 62, 349-355.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.73 paper production potential use in pulp bleaching, application of recombinant FAEA in combination with laccase and xylanase for efficient delignification of wheat straw pulp Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 expressed in Aspergillus niger strain D15-26 (pyrg-) Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.75
-
 methyl ferulate
-
 Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular
-
 Aspergillus niger
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 36000
-
 x * 36000, SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 feruloyl polysaccharide + H2O Aspergillus niger involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?
3.1.1.73 hemicellulose + H2O Aspergillus niger hydrolysis of feruloylated oligosaccharides and diferulate cross-links ferulic acid + lignin + pectin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus niger
-
 glucose as carbon source yields a higher activity than maltose
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 recombinant enzyme Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Aspergillus niger ferulic acid + ?
-
 ?
3.1.1.73 hemicellulose + H2O hydrolysis of feruloylated oligosaccharides and diferulate cross-links Aspergillus niger ferulic acid + lignin + pectin + ?
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Aspergillus niger ferulic acid + methanol
-
 ?
3.1.1.73 methyl sinapinate + H2O
-
 Aspergillus niger sinapic acid + methanol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 36000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 55
-
-
 Aspergillus niger

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.73 Aspergillus niger isoelectric focusing
-
 3.3