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Literature summary extracted from

  • Shapir, N.; Cheng, G.; Sadowsky, M.J.; Wackett, L.P.
    Purification and characterization of TrzF: biuret hydrolysis by allophanate hydrolase supports growth (2006), Appl. Environ. Microbiol., 72, 2491-2495.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.54 gene trzF, overexpression of His-tagged enzyme in presence of a chaperone in Escherichia coli strain BL21(DE3) Enterobacter cloacae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.54 phenyl phosphorodiamidate
-
 Enterobacter cloacae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.54 0.68
-
 allophanate pH 7.4, 25°C, recombinant enzyme Enterobacter cloacae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.54 additional information the enzyme contains no metal ions Enterobacter cloacae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.54 65401
-
 2 * 68000, His-tagged recombinant enzyme, SDS-PAGE, 2 * 65401, sequence calculation Enterobacter cloacae
3.5.1.54 68000
-
 2 * 68000, His-tagged recombinant enzyme, SDS-PAGE, 2 * 65401, sequence calculation Enterobacter cloacae
3.5.1.54 144000
-
 His-tagged recombinant enzyme, gel filtration Enterobacter cloacae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.54 allophanate + H2O Enterobacter cloacae
-
 NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O Enterobacter cloacae 99
-
 NH3 + CO2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.54 Enterobacter cloacae Q9ALV2 gene trzF
-
3.5.1.54 Enterobacter cloacae 99 Q9ALV2 gene trzF
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.54 9.3
-
 purified recombinant enzyme Enterobacter cloacae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.54 allophanate + H2O
-
 Enterobacter cloacae NH3 + CO2
-
 ?
3.5.1.54 allophanate + H2O
-
 Enterobacter cloacae NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O
-
 Enterobacter cloacae 99 NH3 + CO2
-
 ?
3.5.1.54 allophanate + H2O
-
 Enterobacter cloacae 99 NH3 + CO2
-
 ir
3.5.1.54 biuret + H2O
-
 Enterobacter cloacae ?
-
 ?
3.5.1.54 biuret + H2O
-
 Enterobacter cloacae 99 ?
-
 ?
3.5.1.54 malonamic acid + H2O
-
 Enterobacter cloacae ?
-
 ?
3.5.1.54 malonamic acid + H2O
-
 Enterobacter cloacae 99 ?
-
 ?
3.5.1.54 malonamide + H2O
-
 Enterobacter cloacae ?
-
 ?
3.5.1.54 malonamide + H2O
-
 Enterobacter cloacae 99 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.54 dimer 2 * 68000, His-tagged recombinant enzyme, SDS-PAGE, 2 * 65401, sequence calculation Enterobacter cloacae

Synonyms

EC Number Synonyms Comment Organism
3.5.1.54 AtzF
-
 Enterobacter cloacae
3.5.1.54 TrzF
-
 Enterobacter cloacae
3.5.1.54 urea amidolyase
-
 Enterobacter cloacae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.54 25
-
 assay at Enterobacter cloacae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.54 6
-
 allophanate pH 7.4, 25°C, recombinant enzyme Enterobacter cloacae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.54 7.4
-
 assay at Enterobacter cloacae