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Literature summary extracted from

  • Machielsen, R.; Uria, A.R.; Kengen, S.W.; van der Oost, J.
    Production and characterization of a thermostable alcohol dehydrogenase that belongs to the aldo-keto reductase uperfamily (2006), Appl. Environ. Microbiol., 72, 233-238.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 expression in Escherichia coli Pyrococcus furiosus
1.1.1.4 expression in Escherichia coli Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.097
-
 NADH pH 6.1, 70°C Pyrococcus furiosus
1.1.1.1 0.6
-
 NAD+ pH 8.8, 70°C Pyrococcus furiosus
1.1.1.1 6.5
-
 acetoin reduction reaction, pH 6.1, 70°C Pyrococcus furiosus
1.1.1.1 86.8
-
 Butan-2,3-diol pH 8.8, 70°C Pyrococcus furiosus
1.1.1.4 0.097
-
 NADH
-
 Pyrococcus furiosus
1.1.1.4 0.6
-
 NAD+
-
 Pyrococcus furiosus
1.1.1.4 6.5
-
 acetoin
-
 Pyrococcus furiosus
1.1.1.4 86.8
-
 2,3-Butanediol
-
 Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 30000
-
 gel filtration Pyrococcus furiosus
1.1.1.1 32000
-
 1 * 32000, SDS-PAGE Pyrococcus furiosus
1.1.1.4 30000
-
 gel filtration Pyrococcus furiosus
1.1.1.4 32000
-
 1 * 32000, SDS-PAGE Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Pyrococcus furiosus Q8TZM9
-
-
1.1.1.4 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.4 recombinant Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 2 2.5 substrate diacetyl-acetoin, maximal specific activity detected in reduction reaction, 70°C, pH 6.1 Pyrococcus furiosus
1.1.1.1 108
-
 substrate butan-2,3-diol, maximal specific activity detected in oxidation reaction, 70°C, pH 8.8 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 acetoin + NAD+ 18% of the activity with 2,3-butanediol Pyrococcus furiosus diacetyl + NADH
-
 ?
1.1.1.1 acetoin + NADH + H+
-
 Pyrococcus furiosus butan-2,3-diol + NAD+
-
 ?
1.1.1.1 butan-2,3-diol + NAD+
-
 Pyrococcus furiosus acetoin + NADH + H+
-
 ?
1.1.1.1 cyclohexanol + NAD+ 12% of the activity with 2,3-butanediol Pyrococcus furiosus cyclohexanone + NADH + H+
-
 r
1.1.1.1 cyclohexanone + NADH + H+ 38% of the activity with acetoin Pyrococcus furiosus cyclohexanol + NAD+
-
 r
1.1.1.1 D-arabinose + NADH + H+ 66% of the activity with 2,3-butanediol Pyrococcus furiosus D-arabitol + NAD+
-
 ?
1.1.1.1 diacetyl + NADH + H+ 150% of the activity with acetoin Pyrococcus furiosus acetoin + NAD+
-
 ?
1.1.1.1 dihydroxyacetone + NADH + H+ 36% of the activity with acetoin Pyrococcus furiosus glycerol + NAD+
-
 ?
1.1.1.1 dihydroxyacetone phosphate + NADH + H+ 82% of the activity with acetoin Pyrococcus furiosus glycerol phosphate + NAD+
-
 ?
1.1.1.1 DL-glyceraldehyde + H+ 31% of the activity with acetoin Pyrococcus furiosus glycerol + NAD+
-
 ?
1.1.1.1 hexanal + NADH + H+ 16% of the activity with acetoin Pyrococcus furiosus hexan-1-ol + NAD+
-
 ?
1.1.1.1 additional information broad substrate specificity with a preference for the reduction of ketones and the oxidation of secondary alcohols Pyrococcus furiosus ?
-
 ?
1.1.1.1 pentan-2-ol + NAD+
-
 Pyrococcus furiosus pentan-2-one + NADH + H+ 9% of the activity with 2,3-butanediol ?
1.1.1.1 pyruvaldehyde + NADH + H+ 81% of the activity with acetoin Pyrococcus furiosus lactaldehyde + NAD+
-
 ?
1.1.1.4 2,3-butanediol + NAD+
-
 Pyrococcus furiosus acetoin + NADH + H+
-
 r
1.1.1.4 2-pentanone + NADH + H+
-
 Pyrococcus furiosus (S)-2-pentanol + NAD+
-
 ?
1.1.1.4 acetoin + NAD+ 18% of the activity with 2,3-butanediol, in the reverse reaction 150% of the activity with acetoin Pyrococcus furiosus diacetyl + NADH
-
 r
1.1.1.4 cyclohexanone + NADH 38% of the activity with acetoin Pyrococcus furiosus ?
-
 ?
1.1.1.4 dihydroxyacetone + NADH 36% of the activity with acetoin Pyrococcus furiosus ?
-
 ?
1.1.1.4 dihydroxyacetone phosphate + NADH 82% of the activity with acetoin Pyrococcus furiosus ?
-
 ?
1.1.1.4 pyruvic aldehyde + NADH 81% of the activity with acetoin Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 monomer 1 * 32000, SDS-PAGE Pyrococcus furiosus
1.1.1.4 monomer 1 * 32000, SDS-PAGE Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 AdhD
-
 Pyrococcus furiosus
1.1.1.1 PF1960
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 100
-
-
 Pyrococcus furiosus
1.1.1.4 100
-
 catalytic activity increases up to Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 100
-
 half-life 130 min Pyrococcus furiosus
1.1.1.4 100
-
 half-life: 130 min Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 6.1
-
 reduction of ketones Pyrococcus furiosus
1.1.1.1 8.8
-
 oxidation of secondary alcohols Pyrococcus furiosus
1.1.1.4 6.1
-
 reduction of ketones Pyrococcus furiosus
1.1.1.4 8.8
-
 oxidation of secondary alcohols Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+ preferred over NADP+ Pyrococcus furiosus
1.1.1.1 NADP+ 14% of the activity with NAD+ Pyrococcus furiosus
1.1.1.4 NAD+ can use NAD(H) and NADP(H) as cofactor, clear preference for NADH Pyrococcus furiosus
1.1.1.4 NADH can use NAD(H) and NADP(H) as cofactor, clear preference for NADH Pyrococcus furiosus
1.1.1.4 NADP+ 14% of the activity with NAD+ Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.1 0.7
-
 Butan-2,3-diol pH 8.8, 70°C Pyrococcus furiosus