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Literature summary extracted from

  • Lee, H.S.; Kim, Y.J.; Bae, S.S.; Jeon, J.H.; Lim, J.K.; Jeong, B.C.; Kang, S.G.; Lee, J.H.
    Cloning, expression, and characterization of aminopeptidase P from the hyperthermophilic archaeon Thermococcus sp. strain NA1 (2006), Appl. Environ. Microbiol., 72, 1886-1890.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.9
-
 Thermococcus onnurineus NA1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.9 0.96
-
 L-Met-L-Pro pH 5.0, 80°C Thermococcus onnurineus NA1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.9 Co2+ or Mn2+, Zn2+, required. Maximal activity at 3 mM Thermococcus onnurineus NA1
3.4.11.9 Mn2+ or Co2+, Zn2+, required. Maximal activity at 20 mM Thermococcus onnurineus NA1
3.4.11.9 Zn2+ or Mn2+, Co2+, required. Maximal activity at 0.4 mM Thermococcus onnurineus NA1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.9 39714
-
 x * 39714, calculated Thermococcus onnurineus NA1

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.9 Thermococcus onnurineus NA1 Q2QC92 expressed in Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.9 Abz-L-Lys-L-Pro-L-Pro-4-nitroanilide + H2O
-
 Thermococcus onnurineus NA1 Abz-L-Lys + L-Pro-L-Pro-4-nitroanilide
-
 ?
3.4.11.9 L-Met-L-Pro + H2O
-
 Thermococcus onnurineus NA1 L-Met + L-Pro
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.9 ? x * 39714, calculated Thermococcus onnurineus NA1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.9 100
-
-
 Thermococcus onnurineus NA1

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.11.9 40
-
 less than 10% of maximum activity Thermococcus onnurineus NA1
3.4.11.9 50
-
 less than 10% of maximum activity Thermococcus onnurineus NA1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.9 80
-
 half-life above 100 min Thermococcus onnurineus NA1
3.4.11.9 90
-
 half-life 90°C Thermococcus onnurineus NA1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.9 541
-
 L-Met-L-Pro pH 5.0, 80°C Thermococcus onnurineus NA1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.9 5
-
 substrate L-Met-L-Pro Thermococcus onnurineus NA1
3.4.11.9 6.5 7 substrate Abz-L-Lys-L-Pro-L-Pro-4-nitroanilide Thermococcus onnurineus NA1