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Literature summary extracted from
- Improvement of catalytic properties of Escherichia coli penicillin G acylase immobilized on glyoxyl agarose by addition of a six-amino-acid tag (2005), Appl. Environ. Microbiol., 71, 8937-8940.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.1.11 | additional information | addition of a tag of three lysine residues alternating with three glycines to C-terminus of enzyme results in improvement of the immobilization efficiency on glyoxyl agarose and the catalytic protperties of immobilized enzyme, but impairs posttranslational steps of overexpressed protein maturation | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.11 | Escherichia coli | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.11 | 12 | - |
substrate (R)-mandelic acid methyl ester, mutant with three lysine residues alternating with three glycines at C-terminus, pH 8.5, 25°C | Escherichia coli |
| 3.5.1.11 | 12 | - |
substrate (R)-mandelic acid methyl ester, wild-type, pH 8.5, 25°C | Escherichia coli |
| 3.5.1.11 | 12 | - |
substrate 6-nitro-3-[(phenylacetyl)amino]benzoic acid, mutant with three lysine residues alternating with three glycines at C-terminus, pH 8.5, 25°C | Escherichia coli |
| 3.5.1.11 | 16 | - |
substrate 6-nitro-3-[(phenylacetyl)amino]benzoic acid, wild-type, pH 8.5, 25°C | Escherichia coli |
| 3.5.1.11 | 18 | - |
substrate penicillin G, mutant with three lysine residues alternating with three glycines at C-terminus, pH 8.5, 25°C | Escherichia coli |
| 3.5.1.11 | 33 | - |
substrate penicillin G, wild-type, pH 8.5, 25°C | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.11 | (R)-mandelic acid methyl ester + H2O | - |
Escherichia coli | ? | - |
? |  |
| 3.5.1.11 | 6-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O | - |
Escherichia coli | 3-amino-6-nitrobenzoic acid + phenylacetic acid | - |
? | |
| 3.5.1.11 | penicillin G + H2O | - |
Escherichia coli | 6-aminopenicillanate + phenylacetic acid | - |
? | |
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Release 2023.1 (January 2023)
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