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Literature summary extracted from

  • Cheng, G.; Shapir, N.; Sadowsky, M.J.; Wackett, L.P.
    Allophanate hydrolase, not urease, functions in bacterial cyanuric acid metabolism (2005), Appl. Environ. Microbiol., 71, 4437-4445.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.54 sequence analysis and comparison, and phylogenetic analysis Agrobacterium tumefaciens
3.5.1.54 sequence analysis and comparison, and phylogenetic analysis Ralstonia pickettii
3.5.1.54 sequence analysis and comparison, and phylogenetic analysis Herbaspirillum huttiense
3.5.1.54 sequence analysis and comparison, and phylogenetic analysis Enterobacter cloacae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.54 allophanate + H2O Agrobacterium tumefaciens the enzyme is involved in bacterial cyanuric acid metabolism NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O Ralstonia pickettii the enzyme is involved in bacterial cyanuric acid metabolism NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O Pseudomonas sp. the enzyme is involved in bacterial cyanuric acid metabolism NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O Herbaspirillum huttiense the enzyme is involved in bacterial cyanuric acid metabolism NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O Enterobacter cloacae the enzyme is involved in bacterial cyanuric acid metabolism NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O Herbaspirillum huttiense NRRL B-12228 the enzyme is involved in bacterial cyanuric acid metabolism NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O Agrobacterium tumefaciens J14a the enzyme is involved in bacterial cyanuric acid metabolism NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O Ralstonia pickettii D the enzyme is involved in bacterial cyanuric acid metabolism NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O Enterobacter cloacae 99 the enzyme is involved in bacterial cyanuric acid metabolism NH3 + CO2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.54 Agrobacterium tumefaciens
-
 gene atzF
-
3.5.1.54 Agrobacterium tumefaciens J14a
-
 gene atzF
-
3.5.1.54 Enterobacter cloacae Q9ALV2
-
-
3.5.1.54 Enterobacter cloacae 99 Q9ALV2
-
-
3.5.1.54 Herbaspirillum huttiense
-
 gene atzF
-
3.5.1.54 Herbaspirillum huttiense NRRL B-12228
-
 gene atzF
-
3.5.1.54 Pseudomonas sp. Q936X2 gene atzF
-
3.5.1.54 Ralstonia pickettii
-
 gene atzF
-
3.5.1.54 Ralstonia pickettii D
-
 gene atzF
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.54 allophanate + H2O
-
 Agrobacterium tumefaciens NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O
-
 Ralstonia pickettii NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O
-
 Pseudomonas sp. NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O
-
 Herbaspirillum huttiense NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O
-
 Enterobacter cloacae NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O the enzyme is involved in bacterial cyanuric acid metabolism Agrobacterium tumefaciens NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O the enzyme is involved in bacterial cyanuric acid metabolism Ralstonia pickettii NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O the enzyme is involved in bacterial cyanuric acid metabolism Pseudomonas sp. NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O the enzyme is involved in bacterial cyanuric acid metabolism Herbaspirillum huttiense NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O the enzyme is involved in bacterial cyanuric acid metabolism Enterobacter cloacae NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O
-
 Herbaspirillum huttiense NRRL B-12228 NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O the enzyme is involved in bacterial cyanuric acid metabolism Herbaspirillum huttiense NRRL B-12228 NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O
-
 Agrobacterium tumefaciens J14a NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O the enzyme is involved in bacterial cyanuric acid metabolism Agrobacterium tumefaciens J14a NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O
-
 Ralstonia pickettii D NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O the enzyme is involved in bacterial cyanuric acid metabolism Ralstonia pickettii D NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O
-
 Enterobacter cloacae 99 NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O the enzyme is involved in bacterial cyanuric acid metabolism Enterobacter cloacae 99 NH3 + CO2
-
 ir
3.5.1.54 additional information allophanate also shows nonenzymatic decomposition, half-life at pH 8.0 is 50 h Pseudomonas sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.54 AtzF
-
 Agrobacterium tumefaciens
3.5.1.54 AtzF
-
 Ralstonia pickettii
3.5.1.54 AtzF
-
 Pseudomonas sp.
3.5.1.54 AtzF
-
 Herbaspirillum huttiense
3.5.1.54 AtzF
-
 Enterobacter cloacae
3.5.1.54 TrzF
-
 Enterobacter cloacae
3.5.1.54 urea amidolyase
-
 Enterobacter cloacae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.54 30
-
 assay at Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.54 7.5
-
 assay at Pseudomonas sp.