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Literature summary extracted from

  • Nomura, C.T.; Taguchi, K.; Gan, Z.; Kuwabara, K.; Tanaka, T.; Takase, K.; Doi, Y.
    Expression of 3-ketoacyl-acyl carrier protein reductase (fabG) genes enhances production of polyhydroxyalkanoate copolymer from glucose in recombinant Escherichia coli JM109 (2005), Appl. Environ. Microbiol., 71, 4297-4306.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.100 gene fabG, overexpression of His-tagged enzyme in strain BL21(DE3) Escherichia coli
1.1.1.100 gene fabG, overexpression of His-tagged enzyme in strain BL21(DE3), recombinant expression in Escherichia coli strain JM109 leads to enhanced production of polyhydroxyalkanoate, PHA, copolymer from glucose, PHA composition, overview Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.100 Escherichia coli
-
 gene fabG
-
1.1.1.100 Pseudomonas sp. Q4AE87 strain 61-3, non-pathogenic strain from soil, gene fabG
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.100 recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography Escherichia coli
1.1.1.100 recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.100 3-oxodecanoyl-CoA + NADPH
-
 Escherichia coli (R)-3-hydroxydecanoyl-CoA + NADP+
-
 ?
1.1.1.100 3-oxodecanoyl-CoA + NADPH
-
 Pseudomonas sp. (R)-3-hydroxydecanoyl-CoA + NADP+
-
 ?
1.1.1.100 3-oxododecanoyl-CoA + NADPH
-
 Escherichia coli (R)-3-hydroxydodecanoyl-CoA + NADP+
-
 ?
1.1.1.100 3-oxododecanoyl-CoA + NADPH
-
 Pseudomonas sp. (R)-3-hydroxydodecanoyl-CoA + NADP+
-
 ?
1.1.1.100 3-oxohexanoyl-CoA + NADPH
-
 Escherichia coli (R)-3-hydroxyhexanoyl-CoA + NADP+
-
 ?
1.1.1.100 3-oxohexanoyl-CoA + NADPH
-
 Pseudomonas sp. (R)-3-hydroxyhexanoyl-CoA + NADP+
-
 ?
1.1.1.100 3-oxooctanoyl-CoA + NADPH
-
 Escherichia coli (R)-3-hydroxyoctanoyl-CoA + NADP+
-
 ?
1.1.1.100 3-oxooctanoyl-CoA + NADPH
-
 Pseudomonas sp. (R)-3-hydroxyoctanoyl-CoA + NADP+
-
 ?
1.1.1.100 acetoacetyl-CoA + NADPH
-
 Escherichia coli 3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.100 acetoacetyl-CoA + NADPH
-
 Pseudomonas sp. 3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.100 additional information FabG substrate specificity overview Escherichia coli ?
-
 ?
1.1.1.100 additional information FabG substrate specificity overview Pseudomonas sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.100 3-ketoacyl-acyl carrier protein reductase
-
 Escherichia coli
1.1.1.100 3-ketoacyl-acyl carrier protein reductase
-
 Pseudomonas sp.
1.1.1.100 FabG
-
 Escherichia coli
1.1.1.100 FabG
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.100 22
-
 assay at room temperature Escherichia coli
1.1.1.100 22
-
 assay at room temperature Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.100 8
-
 assay at Escherichia coli
1.1.1.100 8
-
 assay at Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.100 NADPH
-
 Escherichia coli
1.1.1.100 NADPH
-
 Pseudomonas sp.