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Literature summary extracted from
- Cloning, overexpression, and characterization of a novel thermostable penicillin G acylase from Achromobacter xylosoxidans: probing the molecular basis for its high thermostability (2004), Appl. Environ. Microbiol., 70, 2764-2770.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.11 | - |
Achromobacter xylosoxidans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.11 | phenylmethylsulfonyl fluoride | strong | Achromobacter xylosoxidans |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.11 | 0.0089 | - |
penicillin G | pH 8.0, 37°C | Achromobacter xylosoxidans | |
| 3.5.1.11 | 0.027 | - |
6-nitro-3-[(phenylacetyl)amino]benzoic acid | pH 8.0, 37°C | Achromobacter xylosoxidans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.11 | 27000 | - |
x * 27000, alpha-subunit, + x * 62400, beta-subunit, SDS-PAGE | Achromobacter xylosoxidans |
| 3.5.1.11 | 62400 | - |
x * 27000, alpha-subunit, + x * 62400, beta-subunit, SDS-PAGE | Achromobacter xylosoxidans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.11 | Achromobacter xylosoxidans | Q83YY8 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.11 | 6-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O | - |
Achromobacter xylosoxidans | 3-amino-6-nitrobenzoic acid + phenylacetic acid | - |
? | |
| 3.5.1.11 | penicillin G + H2O | - |
Achromobacter xylosoxidans | 6-aminopenicillanate + phenylacetic acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.11 | ? | x * 27000, alpha-subunit, + x * 62400, beta-subunit, SDS-PAGE | Achromobacter xylosoxidans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.11 | PGA650 | - |
Achromobacter xylosoxidans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.11 | 60 | - |
- |
Achromobacter xylosoxidans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.11 | 55 | - |
half-life of inactivation 55 min | Achromobacter xylosoxidans |
| 3.5.1.11 | 60 | - |
half-life of inactivation 8 min | Achromobacter xylosoxidans |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.11 | 2.1 | - |
penicillin G | pH 8.0, 37°C | Achromobacter xylosoxidans | |
| 3.5.1.11 | 68.8 | - |
6-nitro-3-[(phenylacetyl)amino]benzoic acid | pH 8.0, 37°C | Achromobacter xylosoxidans | |
| 3.5.1.11 | 72.7 | - |
penicillin G | pH 8.0, 37°C | Achromobacter xylosoxidans | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.11 | 8.5 | - |
- |
Achromobacter xylosoxidans |
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