Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lin, L.L.; Hsu, W.H.; Hsu, W.Y.; Kan, S.C.; Hu, H.Y.
    Phylogenetic analysis and biochemical characterization of a thermostable dihydropyrimidinase from alkaliphilic Bacillus sp. TS-23 (2005), Antonie van Leeuwenhoek, 88, 189-197.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.2 overexpression of His6-tagged DHP in Escherichia coli Novablue cells Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.2 2.4
-
dihydrouracil
-
Bacillus sp. (in: Bacteria)
3.5.2.2 5
-
hydantoin
-
Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.2 Co2+ high stimulation, stimulates enzyme activity most effectively Bacillus sp. (in: Bacteria)
3.5.2.2 Cu2+ low stimulation Bacillus sp. (in: Bacteria)
3.5.2.2 Mn2+ high stimulation Bacillus sp. (in: Bacteria)
3.5.2.2 additional information no stimulation with Mg2+, Ni2+, Ca2+ and Fe2+ Bacillus sp. (in: Bacteria)
3.5.2.2 Zn2+ low stimulation Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.2 52000
-
SDS-PAGE corresponding well with calculated mass of the affinity-tagged translational product of the dhp gene Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.1 Brevibacillus agri
-
-
-
1.3.1.1 Oryza sativa Q6Z744
-
-
1.3.1.1 Salmonella enterica subsp. enterica serovar Typhimurium Q8ZNL7 LT2
-
3.5.2.2 Agrobacterium tumefaciens Q44184
-
-
3.5.2.2 Bacillus sp. (in: Bacteria) Q56S49 TS-23
-
3.5.2.2 Bacillus sp. (in: Bacteria) TS-23 Q56S49 TS-23
-
3.5.2.2 Geobacillus stearothermophilus Q45515
-
-
3.5.2.2 Pseudomonas putida Q59699
-
-
3.5.2.2 Ralstonia pickettii Q8VTT5
-
-
3.5.2.2 Streptomyces coelicolor O69809
-
-
3.5.2.2 Thermus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2 by immobilized metal affinity chromatography Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.2 0.17
-
EDTA-dialyzed/Co2+-substituted enzyme incubated with hydantoin as substrate Bacillus sp. (in: Bacteria)
3.5.2.2 0.36
-
EDTA-dialyzed enzyme incubated with Fe2+ with DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)
3.5.2.2 0.46
-
EDTA-dialyzed enzyme incubated with Ca2+ with DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)
3.5.2.2 0.49
-
EDTA-dialyzed enzyme incubated with Ni2+ with DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)
3.5.2.2 0.52
-
EDTA-dialyzed enzyme with D,L-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)
3.5.2.2 0.54
-
EDTA-dialyzed enzyme incubated with Mg2+ with DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)
3.5.2.2 0.62
-
EDTA-dialyzed/Co2+-substituted enzyme incubated with DL-5(2-(methylthio)-ethyl)hydantoin as substrate Bacillus sp. (in: Bacteria)
3.5.2.2 0.68
-
EDTA-dialyzed enzyme incubated with Cu2+ with DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)
3.5.2.2 0.73
-
EDTA-dialyzed enzyme incubated with Zn2+ with DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)
3.5.2.2 1.69
-
EDTA-dialyzed/Co2+-substituted enzyme incubated with DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)
3.5.2.2 2.38
-
EDTA-dialyzed enzyme incubated with Mn2+ with DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)
3.5.2.2 3.56
-
EDTA-dialyzed/Co2+-substituted enzyme incubated with dihydrouracil as substrate Bacillus sp. (in: Bacteria)
3.5.2.2 3.6
-
EDTA-dialyzed enzyme incubated with Co2+ with DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)

Storage Stability

EC Number Storage Stability Organism
3.5.2.2 50°C, 10 days Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 5,6-dihydrouracil + H2O highest activity of the EDTA-dialyzed/Co2+-substituted enzyme Bacillus sp. (in: Bacteria) ?
-
?
3.5.2.2 5,6-dihydrouracil + H2O highest activity of the EDTA-dialyzed/Co2+-substituted enzyme Bacillus sp. (in: Bacteria) TS-23 ?
-
?
3.5.2.2 DL-5(2-(methylthio)-ethyl)hydantoin + H2O EDTA-dialyzed/Co2+-substituted enzyme Bacillus sp. (in: Bacteria) ?
-
?
3.5.2.2 DL-5(2-(methylthio)-ethyl)hydantoin + H2O EDTA-dialyzed/Co2+-substituted enzyme Bacillus sp. (in: Bacteria) TS-23 ?
-
?
3.5.2.2 DL-p-hydroxyphenylhydantoin + H2O EDTA-dialyzed/Co2+-substituted enzyme Bacillus sp. (in: Bacteria) ?
-
?
3.5.2.2 DL-p-hydroxyphenylhydantoin + H2O EDTA-dialyzed/Co2+-substituted enzyme Bacillus sp. (in: Bacteria) TS-23 ?
-
?
3.5.2.2 hydantoin + H2O lowest activity of the EDTA-dialyzed/Co2+-substituted enzyme Bacillus sp. (in: Bacteria) ?
-
?
3.5.2.2 hydantoin + H2O lowest activity of the EDTA-dialyzed/Co2+-substituted enzyme Bacillus sp. (in: Bacteria) TS-23 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.2 DHP
-
Thermus sp.
3.5.2.2 DHP
-
Salmonella enterica subsp. enterica serovar Typhimurium
3.5.2.2 DHP
-
Brevibacillus agri
3.5.2.2 DHP
-
Bacillus sp. (in: Bacteria)
3.5.2.2 DHP
-
Geobacillus stearothermophilus
3.5.2.2 DHP
-
Pseudomonas putida
3.5.2.2 DHP
-
Ralstonia pickettii
3.5.2.2 DHP
-
Agrobacterium tumefaciens
3.5.2.2 DHP
-
Streptomyces coelicolor
3.5.2.2 DHP
-
Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.2 60
-
DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.2.2 50 80 DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.2 3.04
-
hydantoin
-
Bacillus sp. (in: Bacteria)
3.5.2.2 6.2
-
dihydrouracil
-
Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.2 8
-
DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.2.2 7 9 DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)