Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hsieh, M.L.; Tseng, M.J.; Tseng, M.C.; Chu, Y.H.
    Identification of a new chromophoric substrate in the library of amino acid p-nitroanilides for continuous assay of VanX, a D,D-dipeptidase essential for vancomycin resistance (2006), Anal. Biochem., 354, 104-110.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.13.22 analysis although catalytically ineffecient, the VanX substrate D-leucine-p-nitroanilide is an alternative substrate for VanX because it can be monitored directly and assayed spectrophotometrically which facilitates the routine analysis of enzyme catalysis and the screening discovery of potential VanX inhibitors. In addition, it is with leucine in its D form that possible activities from other contaminated species (other than VanX) in Escherichia coli JM109 are greatly reduced. Moreover, D-leucine-p-nitroanilide needs essentially no sophisticated synthetic chemistry for preparation Enterococcus faecium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.13.22 expressed as a maltose-binding protein fusion protein in Escherichia coli Enterococcus faecium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.13.22 8.9
-
 D-leucine-p-nitroanilide
-
 Enterococcus faecium
3.4.13.22 8.9
-
 D-Leu-4-nitroanilide pH 7.2, 37°C Enterococcus faecium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.13.22 Zn2+
-
 Enterococcus faecium

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.22 Enterococcus faecium Q06241
-
-
3.4.13.22 Enterococcus faecium Q06241 expressed in Escherichai coli as MBP-fusion protein
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.22 by an amylose affinity column and further separation by the DEAE ion exchange chromatography Enterococcus faecium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.22 D-Ala-D-Ala + H2O
-
 Enterococcus faecium D-Ala + D-Ala
-
 ?
3.4.13.22 D-Leu-4-nitroanilide + H2O specific but catalytically inefficient substrate Enterococcus faecium D-Leu + 4-nitroaniline
-
 ?
3.4.13.22 D-leucine-p-nitroanilide + H2O substrate is discovered by construction of a library containing 35 L- and D-amino acid p-nitroanilides Enterococcus faecium D-leucine + p-nitroaniline
-
 ?
3.4.13.22 D/L-Ala-4-nitroanilide + H2O slight hydrolysis for both isomers Enterococcus faecium ?
-
 ?
3.4.13.22 additional information no substrate: L-Leu-4-nitroanilide Enterococcus faecium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.13.22 VanX
-
 Enterococcus faecium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.13.22 37
-
 assay at Enterococcus faecium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.13.22 0.0102
-
 D-leucine-p-nitroanilide
-
 Enterococcus faecium
3.4.13.22 0.0102
-
 D-Leu-4-nitroanilide pH 7.2, 37°C Enterococcus faecium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.13.22 7.2
-
 assay at Enterococcus faecium