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Literature summary extracted from

  • Ohashi, T.; Cramer, N.; Ishimizu, T.; Hase, S.
    Preparation of UDP-galacturonic acid using UDP-sugar pyrophosphorylase (2006), Anal. Biochem., 352, 182-187.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.7.64 synthesis the enzyme is useful for the highly efficient production of UDP-galacturonic acid for studies on pectin biosynthesis Pisum sativum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.64 expression in Escherichia coli Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.64 0.7
-
 diphosphate pH 7.0, 35°C Pisum sativum
2.7.7.64 1.15
-
 D-galacturonic acid 1-phosphate pH 7.0, 35°C Pisum sativum
2.7.7.64 1.26
-
 UDP-galacturonic acid pH 7.0, 35°C Pisum sativum
2.7.7.64 2.27
-
 UTP pH 7.0, 35°C Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.64 Pisum sativum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.64 UTP + D-galacturonic acid 1-phosphate equilibrium constant is 0.24 Pisum sativum diphosphate + UDP-galacturonic acid
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.64 6
-
-
 Pisum sativum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.64 5 9 pH 5.0: about 75% of maximal activity, pH 9.0: about 50% of maximal activity Pisum sativum