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Literature summary extracted from

  • Golich, F.C.; Sigdel, T.; Breece, R.M.; Detar, L.; Herron, L.R.; Crowder, M.W.
    L-Alanine-p-nitroanilide is not a substrate for VanX (2004), Anal. Biochem., 331, 398-400.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.13.22 0.109
-
 D-Ala-D-Ala pH 8.0, 37°C Enterococcus faecium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.13.22 Zn2+ 0.75 equivalents per protein molecule Enterococcus faecium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.13.22 23200
-
 x * 23200, SDS-PAGE Enterococcus faecium

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.22 Enterococcus faecium
-
 recombinant enzyme, Zn2+-form
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.22 D-Ala-D-Ala + H2O
-
 Enterococcus faecium D-Ala + D-Ala
-
 ?
3.4.13.22 additional information no substrate: L-Ala-4-nitroanilide Enterococcus faecium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.13.22 ? x * 23200, SDS-PAGE Enterococcus faecium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.13.22 156
-
 D-Ala-D-Ala pH 8.0, 37°C Enterococcus faecium