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Literature summary extracted from

  • Negishi, O.; Ozawa, T.; Imagawa, H.
    The role of xanthosine in the biosynthesis of caffeine in coffe plants (1985), Agric. Biol. Chem., 49, 2221-2222.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.158 S-adenosyl-L-methionine + xanthosine Coffea arabica in vivo feeding experiments with radio-labeled substrate, product determination, overview S-adenosyl-L-homocysteine + 7-methylxanthosine
-
ir

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.158 Coffea arabica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.158 native enzyme partially from young leaves by ammonium sulfate fractionation, gel filtration, and ultrafiltration Coffea arabica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.158 callus
-
Coffea arabica
-
2.1.1.158 fruit
-
Coffea arabica
-
2.1.1.158 leaf
-
Coffea arabica
-
2.1.1.158 shoot
-
Coffea arabica
-
2.1.1.158 shoot tip
-
Coffea arabica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.158 S-adenosyl-L-methionine + xanthosine
-
Coffea arabica S-adenosyl-L-homocysteine + 7-methylxanthosine
-
ir
2.1.1.158 S-adenosyl-L-methionine + xanthosine in vivo feeding experiments with radio-labeled substrate, product determination, overview Coffea arabica S-adenosyl-L-homocysteine + 7-methylxanthosine
-
ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.158 20
-
assay at Coffea arabica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.158 7.5
-
assay at Coffea arabica