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Literature summary extracted from

  • McCarthy, A.A.; Biget, L.; Lin, C.; Petiard, V.; Tanksley, S.D.; McCarthy, J.G.
    Cloning, expression, crystallization and preliminary x-ray analysis of the XMT and DXMT N-methyltransferases from Coffea canephora (robusta) (2007), Acta Crystallogr. Sect. F, F63, 304-307.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.158 expression of His-tagged XMT1 in Escherichia coli strain BL21(DE3) Coffea canephora
2.1.1.159 expression of His-tagged DXMT1 in Escherichia coli strain BL21(DE3) Coffea canephora
2.1.1.160 expression of His-tagged DXMT1 in Escherichia coli strain BL21(DE3) Coffea canephora

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.158 purified recombinant wild-type and selenomethionine-labeled XMT, 23-28% PEG 3350, 0.2 M LiCl, 0.1 M Tris-HCl, pH 8.5-8.7, 2 mM DTT, 1 mM S-adenosyl-L-cysteine, and 1 mM xanthosine, 1-3 days, 20°C, X-ray diffraction structure determination and analysis at 2.8-3.0 A resolution Coffea canephora
2.1.1.159 purified recombinant wild-type and selenomethionine-labeled DXMT, 23-28% PEG 3350, 0.2 M LiCl, 0.1 M Tris-HCl, pH 8.5-8.7, 2 mM DTT, 1 mM S-adenosyl-L-cysteine, and 1 mM theobromine, 1-3 days, 20°C, X-ray diffraction structure determination and analysis at 2.5-2.7 A resolution Coffea canephora
2.1.1.160 purified recombinant wild-type and selenomethionine-labeled DXMT, 23-28% PEG 3350, 0.2 M LiCl, 0.1 M Tris-HCl, pH 8.5-8.7, 2 mM DTT, 1 mM S-adenosyl-L-cysteine, and 1 mM theobromine, 1-3 days, 20°C, X-ray diffraction structure determination and analysis at 2.5-2.7 A resolution Coffea canephora

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.158 42000
-
 2 * 42000, about, sequence calculation Coffea canephora
2.1.1.158 80000
-
 about, recombinant enzyme, gel filtration Coffea canephora
2.1.1.159 43400
-
 2 * 43400, about, sequence calculation Coffea canephora
2.1.1.159 80000
-
 about, recombinant enzyme, gel filtration Coffea canephora
2.1.1.160 43400
-
 2 * 43400, about, sequence calculation Coffea canephora
2.1.1.160 80000
-
 about, recombinant enzyme, gel filtration Coffea canephora

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.158 S-adenosyl-L-methionine + xanthosine Coffea canephora
-
 S-adenosyl-L-homocysteine + 7-methylxanthosine
-
 ir
2.1.1.159 additional information Coffea canephora the bifunctional enzyme also catalyzes the reaction of the caffeine synthase, EC 2.1.1.160 ?
-
 ?
2.1.1.159 S-adenosyl-L-methionine + 3,7-dimethylxanthine Coffea canephora
-
 S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
2.1.1.159 S-adenosyl-L-methionine + 7-methylxanthine Coffea canephora
-
 S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?
2.1.1.160 additional information Coffea canephora the bifunctional enzyme also catalyzes the reaction of the theobromine synthase, EC 2.1.1.159 ?
-
 ?
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine Coffea canephora
-
 S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.158 Coffea canephora A4GE69 XMT1; var. Robusta
-
2.1.1.159 Coffea canephora A4GE70 DXMT1; var. robusta, bifunctional enzyme
-
2.1.1.160 Coffea canephora A4GE70 DXMT1; var. robusta, bifunctional enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.158 recombinant His-tagged XMT1 from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, cleavage of the His-tag with tobacco etch virus, TEV, protease, followed by gel filtration Coffea canephora
2.1.1.159 recombinant His-tagged DXMT1 from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, cleavage of the His-tag with tobacco etch virus, TEV, protease, followed by gel filtration Coffea canephora
2.1.1.160 recombinant His-tagged DXMT1 from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, cleavage of the His-tag with tobacco etch virus, TEV, protease, followed by gel filtration Coffea canephora

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.158 S-adenosyl-L-methionine + xanthosine
-
 Coffea canephora S-adenosyl-L-homocysteine + 7-methylxanthosine
-
 ir
2.1.1.159 additional information the bifunctional enzyme also catalyzes the reaction of the caffeine synthase, EC 2.1.1.160 Coffea canephora ?
-
 ?
2.1.1.159 S-adenosyl-L-methionine + 3,7-dimethylxanthine
-
 Coffea canephora S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
2.1.1.159 S-adenosyl-L-methionine + 7-methylxanthine
-
 Coffea canephora S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?
2.1.1.160 additional information the bifunctional enzyme also catalyzes the reaction of the theobromine synthase, EC 2.1.1.159 Coffea canephora ?
-
 ?
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine
-
 Coffea canephora S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?

Subunits

EC Number Subunits Comment Organism
2.1.1.158 dimer 2 * 42000, about, sequence calculation Coffea canephora
2.1.1.159 dimer 2 * 43400, about, sequence calculation Coffea canephora
2.1.1.160 dimer 2 * 43400, about, sequence calculation Coffea canephora

Synonyms

EC Number Synonyms Comment Organism
2.1.1.158 XMT1
-
 Coffea canephora
2.1.1.159 DXMT1
-
 Coffea canephora
2.1.1.160 DXMT1
-
 Coffea canephora