Literature summary extracted from
Higashi, N.; Matsuura, T.; Nakagawa, A.; Ishikawa, K.
Crystallization and preliminary X-ray analysis of hyperthermophilic L-threonine dehydrogenase from the archaeon Pyrococcus horikoshii (2005), Acta crystallogr. Sect. F, 61, 432-434.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.1.1.103 |
orf PH0655, expression in Escherichia coli |
Pyrococcus horikoshii |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.1.1.103 |
purified recombinant enzyme, hanging drop vapour diffusion method, 4°C, 10 mg/ml protein in 50 mM Tris-HCl, pH 7.5, 0.0015 ml of protein and reservoir solution are mixed, the reservoir solution contains 0.2 M NaCl, 0.1 M HEPES, pH 7.5, and 40% v/v PEG 400, 5 days, X-ray diffraction structure determination and preliminary analysis at 2.2 A resolution |
Pyrococcus horikoshii |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.1.1.103 |
Zn2+ |
required |
Pyrococcus horikoshii |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.1.1.103 |
37700 |
- |
x * 37700, recombinant enzyme, SDS-PAGE |
Pyrococcus horikoshii |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.1.1.103 |
Pyrococcus horikoshii |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.1.1.103 |
recombinant enzyme from Escherichia coli |
Pyrococcus horikoshii |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.1.1.103 |
additional information |
the enzyme is an NAD+-dependent zinc-containing medium-chain enzyme |
Pyrococcus horikoshii |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.1.1.103 |
? |
x * 37700, recombinant enzyme, SDS-PAGE |
Pyrococcus horikoshii |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.1.1.103 |
L-threonine dehydrogenase |
- |
Pyrococcus horikoshii |
1.1.1.103 |
TDH |
- |
Pyrococcus horikoshii |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.1.1.103 |
additional information |
- |
hyperthermophilic enzyme |
Pyrococcus horikoshii |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.1.1.103 |
NAD+ |
dependent on |
Pyrococcus horikoshii |
|