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Literature summary extracted from

  • Higashi, N.; Matsuura, T.; Nakagawa, A.; Ishikawa, K.
    Crystallization and preliminary X-ray analysis of hyperthermophilic L-threonine dehydrogenase from the archaeon Pyrococcus horikoshii (2005), Acta crystallogr. Sect. F, 61, 432-434.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.103 orf PH0655, expression in Escherichia coli Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.103 purified recombinant enzyme, hanging drop vapour diffusion method, 4°C, 10 mg/ml protein in 50 mM Tris-HCl, pH 7.5, 0.0015 ml of protein and reservoir solution are mixed, the reservoir solution contains 0.2 M NaCl, 0.1 M HEPES, pH 7.5, and 40% v/v PEG 400, 5 days, X-ray diffraction structure determination and preliminary analysis at 2.2 A resolution Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.103 Zn2+ required Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.103 37700
-
x * 37700, recombinant enzyme, SDS-PAGE Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.103 Pyrococcus horikoshii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.103 recombinant enzyme from Escherichia coli Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.103 additional information the enzyme is an NAD+-dependent zinc-containing medium-chain enzyme Pyrococcus horikoshii ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.103 ? x * 37700, recombinant enzyme, SDS-PAGE Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.103 L-threonine dehydrogenase
-
Pyrococcus horikoshii
1.1.1.103 TDH
-
Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.103 additional information
-
hyperthermophilic enzyme Pyrococcus horikoshii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.103 NAD+ dependent on Pyrococcus horikoshii