Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Safo, M.K.; Musayev, F.N.; Schirch, V.
    Structure of Escherichia coli pyridoxine 5-phosphate oxidase in a tetragonal crystal form: insights into the mechanistic pathway of the enzyme (2005), Acta Crystallogr. Sect. D, 61, 599-604.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.3.5 5 mg/ml purified native enzyme with FMN bound in 100 mM K2PO4, pH 7.5, and 5 mM 2-mercaptoethanol, mixed with an equal volume of reservoir solution containing 60 mM HEPES, pH 6.8, and 0.65 M KH2PO4/NH4H2PO4, formation of tetragonal crystals within 4-5 weeks, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular-replacement method Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.5 pyridoxamine 5'-phosphate + FMN + H2O + 2 H+ Escherichia coli
-
 pyridoxal 5'-phosphate + FMNH2 + NH3
-
 ?
1.4.3.5 pyridoxine 5'-phosphate + FMN Escherichia coli
-
 pyridoxal 5'-phosphate + FMNH2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.5 Escherichia coli P0AFI7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.5 native enzyme with tightly bound FMN Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2 reaction mechanism, the oxidation involves a stereospecific hydride-ion transfer between C4' of pyridoxal 5'-phosphate and N5 of FMN, structure-function relationship, substrate binding and active site structure Escherichia coli
a
1.4.3.5 pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2 reaction mechanism, the oxidation involves a stereospecific hydride-ion transfer between C4' of pyridoxal 5'-phosphate and N5 of FMN, structure-function relationship, substrate binding and active site structure Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.5 pyridoxamine 5'-phosphate + FMN + H2O + 2 H+
-
 Escherichia coli pyridoxal 5'-phosphate + FMNH2 + NH3
-
 ?
1.4.3.5 pyridoxine 5'-phosphate + FMN
-
 Escherichia coli pyridoxal 5'-phosphate + FMNH2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.5 ePNPOx
-
 Escherichia coli
1.4.3.5 pyridoxine 5-phosphate oxidase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.5 FMN
-
 Escherichia coli