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Literature summary extracted from
- Influence of dipeptidyl peptidase IV on enzymatic properties of adenosine deaminase (2006), Acta Biochim. Pol., 53, 539-546.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.4 | 1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine | i.e. 1-dEHNA | Bos taurus |  |
| 3.5.4.4 | 1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine | i.e. 1-dEHNA | Homo sapiens | |
| 3.5.4.4 | 1-deazaadenosine | - |
Bos taurus | |
| 3.5.4.4 | 1-deazaadenosine | - |
Homo sapiens | |
| 3.5.4.4 | 3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine | i.e. 3-dEHNA | Bos taurus | |
| 3.5.4.4 | 3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine | i.e. 3-dEHNA | Homo sapiens | |
| 3.5.4.4 | 3-deazaadenosine | - |
Bos taurus | |
| 3.5.4.4 | 3-deazaadenosine | - |
Homo sapiens | |
| 3.5.4.4 | erythro-9-(2-hydroxy-3-nonyl)adenine | i.e. EHNA | Bos taurus | |
| 3.5.4.4 | erythro-9-(2-hydroxy-3-nonyl)adenine | i.e. EHNA | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.4.4 | additional information | - |
additional information | kinetics of the large and the small isozyme | Homo sapiens | |
| 3.5.4.4 | additional information | - |
additional information | kinetics of the large and the small isozyme | Bos taurus | |
| 3.5.4.4 | 0.039 | - |
2'-deoxyadenosine | kidney cortex large isozyme | Bos taurus | |
| 3.5.4.4 | 0.04 | - |
2'-deoxyadenosine | pleural fluid large isozyme | Homo sapiens | |
| 3.5.4.4 | 0.042 | - |
2'-deoxyadenosine | blood serum large isozyme | Homo sapiens | |
| 3.5.4.4 | 0.053 | - |
adenosine | blood serum large isozyme | Homo sapiens | |
| 3.5.4.4 | 0.056 | - |
adenosine | kidney cortex large isozyme | Bos taurus | |
| 3.5.4.4 | 0.07 | - |
adenosine | pleural fluid large isozyme | Homo sapiens | |
| 3.5.4.4 | 0.093 | - |
adenosine | spleen small isozyme | Bos taurus | |
| 3.5.4.4 | 0.129 | - |
adenosine | lung small isozyme | Bos taurus | |
| 3.5.4.4 | 0.135 | - |
2'-deoxyadenosine | spleen small isozyme | Bos taurus | |
| 3.5.4.4 | 0.189 | - |
2'-deoxyadenosine | lung small isozyme | Bos taurus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.4.4 | extracellular | LADA | Homo sapiens | - |
- |
| 3.5.4.4 | membrane | large isozyme LADA | Homo sapiens | 16020 | - |
| 3.5.4.4 | membrane | large isozyme LADA | Bos taurus | 16020 | - |
| 3.5.4.4 | soluble | small subunit SADA | Homo sapiens | - |
- |
| 3.5.4.4 | soluble | small subunit SADA | Bos taurus | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.4 | 2'-deoxyadenosine + H2O | Homo sapiens | - |
2'-deoxyinosine + NH3 | - |
ir | |
| 3.5.4.4 | 2'-deoxyadenosine + H2O | Bos taurus | - |
2'-deoxyinosine + NH3 | - |
ir | |
| 3.5.4.4 | adenosine + H2O | Homo sapiens | - |
inosine + NH3 | - |
ir | |
| 3.5.4.4 | adenosine + H2O | Bos taurus | - |
inosine + NH3 | - |
ir | |
| 3.5.4.4 | additional information | Bos taurus | the enzyme is bound and complexed by the cell membraneous dipeptidyl peptidase IV, which is identical to the activated immune cell antigen CD26, playing a role in the immune system, overview | ? | - |
? | |
| 3.5.4.4 | additional information | Homo sapiens | the large isozyme is bound and complexed by the cell membraneous dipeptidyl peptidase IV, which is identical to the activated immune cell antigen CD26, playing a role in the immune system, overview | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.14.5 | Homo sapiens | - |
- |
- |
| 3.5.4.4 | Bos taurus | - |
small and large isozymes SADA and LADA | - |
| 3.5.4.4 | Homo sapiens | - |
small and large isozymes SADA and LADA | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.4.4 | purification of the soluble large isozyme complexed with dipeptidyl peptidase IV from blood serum and pleural fluid by anion exchange chromatography, gel filtration, and affinity chromatography steps to about 90% purity | Homo sapiens |
| 3.5.4.4 | small isozyme from lung and spleen to homogeneity, purification of the soluble large isozyme complexed with dipeptidyl peptidase IV from kidney cortex by anion exchange chromatography, gel filtration, and affinity chromatography steps to about 90% purity | Bos taurus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.5.4.4 | adenosine + H2O = inosine + NH3 | reaction mechanism, structure-function relationship of ADA and dipeptidyl peptidase IV | Homo sapiens | |
| 3.5.4.4 | adenosine + H2O = inosine + NH3 | reaction mechanism, structure-function relationship of ADA and dipeptidyl peptidase IV | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.4.4 | blood | LADA | Homo sapiens | - |
| 3.5.4.4 | kidney | cortex | Bos taurus | - |
| 3.5.4.4 | lung | SADA | Bos taurus | - |
| 3.5.4.4 | pleural fluid | LADA | Homo sapiens | - |
| 3.5.4.4 | serum | LADA | Homo sapiens | - |
| 3.5.4.4 | spleen | SADA | Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.4 | 0.45 | - |
purified large isozyme from blood serum | Homo sapiens |
| 3.5.4.4 | 0.65 | - |
purified large isozyme from pleural fluid | Homo sapiens |
| 3.5.4.4 | 5.4 | - |
purified small isozyme from kidney cortex | Bos taurus |
| 3.5.4.4 | 350 | - |
purified small isozyme from lung and spleen | Bos taurus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.4.4 | -20°C, purified large subunit, 2 months without loss of activity | Homo sapiens |
| 3.5.4.4 | -20°C, purified large subunit, 2 months without loss of activity | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.14.5 | Gly-Pro-p-nitroanilide + H2O | - |
Homo sapiens | Gly-Pro + p-nitroaniline | - |
? | |
| 3.4.14.5 | additional information | kinetic characteristics of SADA (the catalytic subunit of adenosine deaminase), are modulated by the interaction with DPPIV-CD26 in favour of neutralization of toxic nucleosides (especially 2´-dAdo) in the extracellular medium | Homo sapiens | ? | - |
? | |
| 3.5.4.4 | 2'-deoxyadenosine + H2O | - |
Homo sapiens | 2'-deoxyinosine + NH3 | - |
ir | |
| 3.5.4.4 | 2'-deoxyadenosine + H2O | - |
Bos taurus | 2'-deoxyinosine + NH3 | - |
ir | |
| 3.5.4.4 | adenosine + H2O | - |
Homo sapiens | inosine + NH3 | - |
ir | |
| 3.5.4.4 | adenosine + H2O | - |
Bos taurus | inosine + NH3 | - |
ir | |
| 3.5.4.4 | additional information | the enzyme is bound and complexed by the cell membraneous dipeptidyl peptidase IV, which is identical to the activated immune cell antigen CD26, playing a role in the immune system, overview | Bos taurus | ? | - |
? | |
| 3.5.4.4 | additional information | the large isozyme is bound and complexed by the cell membraneous dipeptidyl peptidase IV, which is identical to the activated immune cell antigen CD26, playing a role in the immune system, overview | Homo sapiens | ? | - |
? | |
| 3.5.4.4 | additional information | substrate specificities of the large and the small isozyme, overview | Homo sapiens | ? | - |
? | |
| 3.5.4.4 | additional information | substrate specificities of the large and the small isozyme, overview | Bos taurus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.4.4 | More | the enzyme exists as large and small isozymes, SADA and LADA, the latter is formed by the catalytic subunit monomer, the first by SADA complexed with the ADA complexing protein, i.e. the dipeptidyl peptidase IV | Homo sapiens |
| 3.5.4.4 | More | the enzyme exists as large and small isozymes, SADA and LADA, the latter is formed by the catalytic subunit monomer, the first by SADA complexed with the ADA complexing protein, i.e. the dipeptidyl peptidase IV | Bos taurus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.14.5 | CD26 | - |
Homo sapiens |
| 3.4.14.5 | dipeptidyl peptidase IV | - |
Homo sapiens |
| 3.4.14.5 | DPPIV | - |
Homo sapiens |
| 3.5.4.4 | ADA | - |
Homo sapiens |
| 3.5.4.4 | ADA | - |
Bos taurus |
| 3.5.4.4 | LADA | - |
Homo sapiens |
| 3.5.4.4 | SADA | - |
Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.4 | 6.5 | 7.6 | large isozyme from kidney cortex | Bos taurus |
| 3.5.4.4 | 6.5 | 7.8 | large isozyme from blood serum | Homo sapiens |
| 3.5.4.4 | 6.5 | 8.1 | small isozyme from lung | Bos taurus |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.4.4 | additional information | - |
additional information | inhibition kinetics of the large and the small isozyme | Bos taurus | |
| 3.5.4.4 | additional information | - |
additional information | inhibition kinetics of the large isozyme | Homo sapiens | |
| 3.5.4.4 | 0.000021 | - |
erythro-9-(2-hydroxy-3-nonyl)adenine | kidney cortex large isozyme | Bos taurus | |
| 3.5.4.4 | 0.000025 | - |
3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine | blood serum large isozyme | Homo sapiens | |
| 3.5.4.4 | 0.00003 | - |
erythro-9-(2-hydroxy-3-nonyl)adenine | pleural fluid large isozyme | Homo sapiens | |
| 3.5.4.4 | 0.000036 | - |
3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine | pleural fluid large isozyme | Homo sapiens | |
| 3.5.4.4 | 0.00004 | - |
erythro-9-(2-hydroxy-3-nonyl)adenine | lung small isozyme | Bos taurus | |
| 3.5.4.4 | 0.00005 | - |
3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine | kidney cortex large isozyme | Bos taurus | |
| 3.5.4.4 | 0.000057 | - |
erythro-9-(2-hydroxy-3-nonyl)adenine | spleen small isozyme | Bos taurus | |
| 3.5.4.4 | 0.00006 | - |
erythro-9-(2-hydroxy-3-nonyl)adenine | blood serum large isozyme | Homo sapiens | |
| 3.5.4.4 | 0.00011 | - |
3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine | spleen small isozyme | Bos taurus | |
| 3.5.4.4 | 0.00012 | - |
3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine | lung small isozyme | Bos taurus | |
| 3.5.4.4 | 0.0004 | - |
1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine | kidney cortex large isozyme | Bos taurus | |
| 3.5.4.4 | 0.00045 | - |
1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine | pleural fluid large isozyme | Homo sapiens | |
| 3.5.4.4 | 0.0006 | - |
1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine | blood serum large isozyme | Homo sapiens | |
| 3.5.4.4 | 0.0011 | - |
1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine | spleen small isozyme | Bos taurus | |
| 3.5.4.4 | 0.0012 | - |
1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine | lung small isozyme | Bos taurus | |
| 3.5.4.4 | 0.0016 | - |
1-deazaadenosine | kidney cortex large isozyme | Bos taurus | |
| 3.5.4.4 | 0.0025 | - |
1-deazaadenosine | pleural fluid large isozyme | Homo sapiens | |
| 3.5.4.4 | 0.0029 | - |
1-deazaadenosine | blood serum large isozyme | Homo sapiens | |
| 3.5.4.4 | 0.0029 | - |
1-deazaadenosine | lung small isozyme | Bos taurus | |
| 3.5.4.4 | 0.0057 | - |
1-deazaadenosine | spleen small isozyme | Bos taurus | |
| 3.5.4.4 | 0.52 | - |
3-deazaadenosine | blood serum large isozyme | Homo sapiens | |
| 3.5.4.4 | 0.69 | - |
3-deazaadenosine | kidney cortex large isozyme | Bos taurus | |
| 3.5.4.4 | 0.81 | - |
3-deazaadenosine | lung small isozyme | Bos taurus | |
| 3.5.4.4 | 1 | - |
3-deazaadenosine | above, pleural fluid large isozyme | Homo sapiens | |
| 3.5.4.4 | 1 | - |
3-deazaadenosine | above, spleen small isozyme | Bos taurus | |
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