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Literature summary extracted from

  • Dubin, G.
    Defense against own arms: staphylococcal cysteine proteases and their inhibitors (2003), Acta Biochim. Pol., 50, 715-724.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.48 ssp and scp operons, genomic organization of ssp and scp genes, overview Staphylococcus epidermidis
3.4.22.48 ssp and scp operons, genomic organization of ssp and scp genes, overview Staphylococcus warneri
3.4.22.48 ssp and scp operons, genomic organization of ssp and scp genes, overview, overexpression of scp operon genes in Escherichia coli Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.22.48 crystal structure determination of staphopain A in complex with inhibitor E64, folding pattern Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.48 additional information inactivation of gene sspA by transposon insertion exertes a polar effect on genes sspB and sspC expression, the inactivation of all three genes results in attenuation of virulence Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.48 E64 ScpA-inhibitor binding structure Staphylococcus aureus
3.4.22.48 additional information staphostin homologue genes are probably encoding enzyme inhibitors Staphylococcus epidermidis
3.4.22.48 additional information staphostin homologue genes are probably encoding enzyme inhibitors Staphylococcus warneri
3.4.22.48 staphostatin A i.e. ScpB, intracellular, endogenous specific inhibitor of ScpA forming noncovalent complexes, structure determination, slight cleaving of the inhibitor by the enzyme Staphylococcus aureus
3.4.22.48 staphostatin B i.e. SspC, intracellular, endogenous specific inhibitor of SspB forming noncovalent complexes, structure determination, slight cleaving of the inhibitor by the enzyme Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.48 extracellular the enzyme is secreted as zymogen Staphylococcus aureus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.48 kininogen + H2O Staphylococcus aureus activation of human protein by SspA, kinin generation is responsible for infection associated pain and endema kinin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.48 Staphylococcus aureus
-
-
-
3.4.22.48 Staphylococcus epidermidis
-
-
-
3.4.22.48 Staphylococcus warneri
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.48 proteolytic modification the enzyme staphopain B is synthesized as preproenzyme, the prefragment is cleaved resulting in the proenzyme, after secretion the proenzyme is proteolytically activated to the mature enzyme Staphylococcus aureus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.22.48 broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate active site structure and topology of ScpA Staphylococcus aureus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.48 alpha1-proteinase inhibitor + H2O human protein, inactivation by SspA Staphylococcus aureus ?
-
 ?
3.4.22.48 Gelatin + H2O staphopain B Staphylococcus aureus ?
-
 ?
3.4.22.48 kininogen + H2O activation of human protein by SspA, kinin generation is responsible for infection associated pain and endema Staphylococcus aureus kinin + ?
-
 ?
3.4.22.48 kininogen + H2O human protein, activation by SspA Staphylococcus aureus kinin + ?
-
 ?
3.4.22.48 additional information staphopains A and B are cysteine proteases, staphopain shows no activity with casein Staphylococcus aureus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.48 SCPA
-
 Staphylococcus aureus
3.4.22.48 SspB
-
 Staphylococcus aureus
3.4.22.48 staphopain A
-
 Staphylococcus aureus
3.4.22.48 staphopain B
-
 Staphylococcus aureus