Literature summary extracted from

Zhang, H.F.; Zheng, B.S.; Peng, Y.; Lou, Z.Y.; Feng, Y.; Rao, Z.H.
Expression, purification and crystal structure of a truncated acylpeptide hydrolase from Aeropyrum pernix K1 (2005), Acta Biochim. Biophys. Sin., 37, 613-617.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.4.19.1	a truncated mutant of apAPH that lacks the first short alpha-helix at the N-terminal is cloned and expressed in Escherichia coli	Aeropyrum pernix K1

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.4.19.1	hanging-drop vapor diffusion method, 2.5 A resolution, space group: P2(1)2(1)2(1), unit cell parameters: a = 63.1 A, b = 102.3 A, c = 163.9 A, truncated mutant of apAPH that lacks the first short alpha-helix at the N-terminal is cloned and expressed in Escherichia coli	Aeropyrum pernix K1

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.4.19.1	DELTA1-21	optimal temperature of a truncated mutant of apAPH that lacks the first short alpha-helix at the N-terminal is decreased by 15°C	Aeropyrum pernix K1

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.19.1	Aeropyrum pernix K1	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.4.19.1	-	Aeropyrum pernix K1

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
3.4.19.1	75	-	truncated mutant of apAPH that lacks the first short alpha-helix at the N-terminal	Aeropyrum pernix K1
3.4.19.1	90	-	wild-type enzyme	Aeropyrum pernix K1

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Release 2023.1 (January 2023)