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Literature summary extracted from

  • Xu, Z.W.; Zhou, H.X.; Huang, W.N.
    Some properties of the allantoate amidohydrolase from French bean seedlings (2004), Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao, 30, 460-468.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.3.9 lysine
-
 Phaseolus vulgaris
3.5.3.9 additional information phenylhydrazine crucial for enzyme activity Phaseolus vulgaris
3.5.3.9 tryptophan
-
 Phaseolus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.9 Co2+ 0.15 mM completely inhibits incubation mixture with Mn2+ Phaseolus vulgaris
3.5.3.9 Cu2+ 0.15 mM completely inhibits incubation mixture with Mn2+ Phaseolus vulgaris
3.5.3.9 EDTA abolishes activation of enzyme by Mn2+; final concentration of 2 mM Phaseolus vulgaris
3.5.3.9 Fe2+ 0.15 mM completely inhibits incubation mixture with Mn2+ Phaseolus vulgaris
3.5.3.9 glycolate 50% activity loss with 0.06 mM glycolate for 10 min at 30°C; strong inhibitor, 50% inhibition at 0.06 mM Phaseolus vulgaris
3.5.3.9 glyoxylate 50% activity loss with 0.02 mM glycolate for 10 min at 30°C; strong inhibitor, 50% inhibition at 0.02 mM Phaseolus vulgaris
3.5.3.9 guanidine 10% and 80% activity loss with 2 M and 5 M L-guanidine pre-treatment for 10 min at 30°C Phaseolus vulgaris
3.5.3.9 iminourea pre-treatment with 2mM or 5mM results in a loss of acitivity of 10% or 80% of activity Phaseolus vulgaris
3.5.3.9 additional information pre-treatment with N-acetylimidazole, iodoacetic acid or 5,5'-dithiobis-(2-nitrobenzoic acid) has no effect on enzyme activity Phaseolus vulgaris
3.5.3.9 N-Acetylimidazole 5.6% activity loss with 10 mM n-acetylimidazole 10 min at 30°C Phaseolus vulgaris
3.5.3.9 N-bromosuccinimide 100% activity loss with 10 mM n-bromosuccinimide for 10 min at 30°C; pre-treatment completely inhibits activity Phaseolus vulgaris
3.5.3.9 pyridoxal 5'-phosphate pre-treatment with 10 mM completely inhibits activity Phaseolus vulgaris
3.5.3.9 pyridoxal-5-phosphate 100% activity loss with 10 mM pyridoxal-5-phosphate for 10 min at 30°C Phaseolus vulgaris
3.5.3.9 SDS 50% activity loss with 50 mM SDS pre-treatment for 10 min at 30°C; pre-treatment with 50 mM results in a loss of acitivity of 50% of activity Phaseolus vulgaris
3.5.3.9 Urea 5% and 20% activity loss with 2 M and 5 M urea pre-treatment for 10 min at 30°C Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.9 0.076
-
 Allantoate
-
 Phaseolus vulgaris
3.5.3.9 76
-
 Allantoate
-
 Phaseolus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.9 Cd2+ can replace Mn2+, 85% of activity measured in the presence of Mn2+ Phaseolus vulgaris
3.5.3.9 Mn2+ no enzyme activity without Mn2+, maximal activity at concentrations between 0.1 and 0.5 mM Phaseolus vulgaris
3.5.3.9 Mn2+ essential for enzyme activity, maximal activity between 0.1-0.5 mM Phaseolus vulgaris
3.5.3.9 additional information Mg2+, Ca2+, Cu2+, Co2+ and Fe2+ can not replace Mn2+ Phaseolus vulgaris
3.5.3.9 Ni2+ can replace Mn2+, 32% of activity measured in the presence of Mn2+ Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.9 13500
-
 gel filtration Phaseolus vulgaris
3.5.3.9 13500
-
 gel filtration on Sephacryl S-200 column Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.9 Phaseolus vulgaris
-
 french bean
-
3.5.3.9 Phaseolus vulgaris
-
 cv. baishui
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.9 579fold purified ond DEAE-Sephadex column, ADE2 pool Phaseolus vulgaris
3.5.3.9 gel filtration with Sephacryl S-200 and Sephadex A50 columns, and dialysis against Tris-HCl pH 7.5 at 4°C Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.3.9 cotyledon
-
 Phaseolus vulgaris
-
3.5.3.9 leaf
-
 Phaseolus vulgaris
-
3.5.3.9 root
-
 Phaseolus vulgaris
-
3.5.3.9 seed
-
 Phaseolus vulgaris
-
3.5.3.9 seedling
-
 Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.9 0.0004
-
 ungerminated seeds Phaseolus vulgaris
3.5.3.9 0.00138
-
 in crude extract after first purification Phaseolus vulgaris
3.5.3.9 0.00138
-
 crude extract from seedlings Phaseolus vulgaris
3.5.3.9 0.0018
-
 6-d-old seedlings Phaseolus vulgaris
3.5.3.9 0.776
-
 579fold purified enzyme from seedlings Phaseolus vulgaris
3.5.3.9 0.8
-
 after 579-fold purification Phaseolus vulgaris
3.5.3.9 1
-
 vmax of partially purified ADE2 pool Phaseolus vulgaris

Storage Stability

EC Number Storage Stability Organism
3.5.3.9 -30°C, 2 months, 5% loss of activity Phaseolus vulgaris
3.5.3.9 -30°C, Tris-HCl buffer, pH 7.5, 2 months Phaseolus vulgaris
3.5.3.9 4°C, 1 week, 10% loss of activity Phaseolus vulgaris
3.5.3.9 4°C, Tris-HCl buffer, pH 7.5, 1 week Phaseolus vulgaris
3.5.3.9 55°C, 15 min, 15% loss of activity Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.9 allantoate + 2 H2O
-
 Phaseolus vulgaris ureidoglycolate + CO2 + 2 NH3
-
 ?
3.5.3.9 allantoate + H2O
-
 Phaseolus vulgaris ureidoglycine + NH3 + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.3.9 ADE2
-
 Phaseolus vulgaris
3.5.3.9 ALAH
-
 Phaseolus vulgaris
3.5.3.9 Allantoate amidinohydrolase
-
 Phaseolus vulgaris
3.5.3.9 allantoate amidohydrolase
-
 Phaseolus vulgaris
3.5.3.9 allantoate amidohydrolase ALAH Phaseolus vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.9 30
-
-
 Phaseolus vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.9 55
-
 treatment for 15 min reveals a loss of 15% of acitivity Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.9 8.5
-
-
 Phaseolus vulgaris
3.5.3.9 8.5
-
 in tricine/NaOH buffer Phaseolus vulgaris