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Literature summary extracted from

  • Greenberg, J.R.; Price, N.P.; Oliver, R.P.; Sherman, F.; Rustchenko, E.
    Candida albicans SOU1 encodes a sorbose reductase required for L-sorbose utilization (2005), Yeast, 22, 957-969.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.289 1873
-
 D-fructose K0.5-value, positive cooperativity Candida albicans
1.1.1.289 3953
-
 L-sorbose pH 7.5, 25°C Candida albicans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.289 31000
-
 4 * 31000, SDS-PAGE and calculated Candida albicans
1.1.1.289 124000
-
 MALDI-TOF Candida albicans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.289 Candida albicans
-
 recombinant enzyme with FLAG-tag
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.289 recombinant enzyme with FLAG-tag Candida albicans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.289 2.93
-
 substrate D-sorbitol, pH 6.2, 25°C Candida albicans
1.1.1.289 33.1
-
 substrate D-fructose, pH 6.2, 25°C Candida albicans
1.1.1.289 145
-
 substrate L-sorbose, pH 6.2, 25°C Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.289 D-fructose + NADPH less effective than reaction with L-sorbose Candida albicans D-mannitol + NADP+
-
 ?
1.1.1.289 D-sorbitol + NADP+ low efficiency Candida albicans L-sorbose + NADPH + H+
-
 ?
1.1.1.289 L-sorbose + NADPH + H+
-
 Candida albicans L-sorbitol + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.289 tetramer 4 * 31000, SDS-PAGE and calculated Candida albicans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.289 65
-
 2 min, more than 90% inactivation Candida albicans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.289 154
-
 D-fructose pH 7.5, 25°C, positive cooperativity Candida albicans
1.1.1.289 782
-
 L-sorbose pH 7.5, 25°C, Michaelis-Menten kinetics Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.289 6.2
-
-
 Candida albicans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.289 NADPH
-
 Candida albicans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.289 Candida albicans isoelectric focusing and calculated
-
 4.8