Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Adam, A.C.; Latorre-Garcia, L.; Polaina, J.
    Structural analysis of glucoamylase encoded by the STA1 gene of Saccharomyces cerevisiae (var. diastaticus) (2004), Yeast, 21, 379-388.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.3 gene STA1, DNA and amino acid sequence determination and analysis, homology modelling Saccharomyces cerevisiae 'var. diastaticus'

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.3 extracellular the enzyme is secreted from the cells Saccharomyces cerevisiae 'var. diastaticus'
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 80000
-
 x * 80000, SDS-PAGE Saccharomyces cerevisiae 'var. diastaticus'
3.2.1.3 300000
-
 native PAGE Saccharomyces cerevisiae 'var. diastaticus'

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.3 starch + H2O Saccharomyces cerevisiae 'var. diastaticus' soluble starch starch + beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Saccharomyces cerevisiae 'var. diastaticus' P04065 gene STA1, different strains
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.3 glycoprotein Sta1p is hyperglycosylized, over 80% carbohydrate content Saccharomyces cerevisiae 'var. diastaticus'

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 partially Saccharomyces cerevisiae 'var. diastaticus'

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 additional information
-
 activity in different strains Saccharomyces cerevisiae 'var. diastaticus'

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 additional information the enzyme binds poorly to insoluble starch Saccharomyces cerevisiae 'var. diastaticus' ?
-
 ?
3.2.1.3 starch + H2O soluble starch Saccharomyces cerevisiae 'var. diastaticus' starch + beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 More structure analysis of Sta1p, modelling Saccharomyces cerevisiae 'var. diastaticus'
3.2.1.3 oligomer x * 80000, SDS-PAGE Saccharomyces cerevisiae 'var. diastaticus'

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 glucoamylase
-
 Saccharomyces cerevisiae 'var. diastaticus'