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Literature summary extracted from
- Adsorption-elution purification of chimeric Bacillus stearothermophilus leucine aminopeptidase II with raw-starch-binding activity (2005), World J. Microbiol. Biotechnol., 21, 689-694.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.11.10 | expression of the chimeric enzyme comprising the raw-starch-binding domain of Bacillus sp. strain TS-23 alpha-amylase anthe Bacillus stearothermophilus LAPII in Escherichia coli strain M15 | Geobacillus stearothermophilus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.11.10 | additional information | construction of a chimeric enzyme comprising the raw-starch-binding domain of Bacillus sp. strain TS-23 alpha-amylase and the Bacillus stearothermophilus LAPII, the chimeric mutant enzyme binds raw starch, overview | Geobacillus stearothermophilus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.10 | additional information | - |
additional information | kinetics of raw starch-binding by the chimeric mutant enzyme | Geobacillus stearothermophilus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.10 | 61000 | - |
x * 61000, recombinant chimeric enzyme, SDS-PAGE | Geobacillus stearothermophilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.10 | Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.10 | recombinant chimeric enzyme 20.4fold from Escherichia coli strain M15 by an affinity adsorption chromatographic step on a raw starch resin, screening of different elution methods, overview | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.10 | 466.7 | - |
purified recombinant enzyme | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.10 | L-Leu-4-nitroanilide + H2O | - |
Geobacillus stearothermophilus | L-Leu + 4-nitroaniline | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.11.10 | ? | x * 61000, recombinant chimeric enzyme, SDS-PAGE | Geobacillus stearothermophilus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.11.10 | LAP | - |
Geobacillus stearothermophilus |
| 3.4.11.10 | leucine aminopeptidase II | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.10 | 60 | - |
assay at | Geobacillus stearothermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.10 | 8 | - |
assay at | Geobacillus stearothermophilus |
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Release 2023.1 (January 2023)
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