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Literature summary extracted from

  • Shibasaki, T.; Sakurai, W.; Hasegawa, A.; Uosaki, Y.; Mori, H.; Yoshida, M.; Ozaki, A.
    Substrate selectivities of proline hydroxylases (1999), Tetrahedron Lett., 40, 5227-5230.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.28 expression in Escherichia coli Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.28 0.2
-
 L-proline
-
 Streptomyces sp.
1.14.11.28 0.4
-
 L-proline
-
 Streptomyces sp.
1.14.11.28 2.1
-
 L-2-azetidinecarboxylic acid
-
 Streptomyces sp.
1.14.11.28 3.8
-
 L-pipecolic acid
-
 Streptomyces sp.
1.14.11.28 8.4
-
 3,4-dehydro-L-proline
-
 Streptomyces sp.
1.14.11.28 100
-
 L-pipecolic acid
-
 Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.28 Streptomyces sp.
-
 strainTH1
-
1.14.11.28 Streptomyces sp. TH1
-
 strainTH1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.28 2 chromatographic steps to homogeneity Streptomyces sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.28 cell culture
-
 Streptomyces sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.28 3,4-dehydro-L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. cis-3,4-epoxy-L-proline + succinate + CO2
-
 ?
1.14.11.28 3,4-dehydro-L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. TH1 cis-3,4-epoxy-L-proline + succinate + CO2
-
 ?
1.14.11.28 L-2-azetidinecarboxylic acid + 2-oxoglutarate + O2
-
 Streptomyces sp. cis-3-hydroxyazetidine-L-2-carboxylic acid + succinate + CO2
-
 ?
1.14.11.28 L-2-azetidinecarboxylic acid + 2-oxoglutarate + O2
-
 Streptomyces sp. TH1 cis-3-hydroxyazetidine-L-2-carboxylic acid + succinate + CO2
-
 ?
1.14.11.28 L-pipecolic acid + 2-oxoglutarate + O2
-
 Streptomyces sp. cis-3-hydroxy-L-pipecolic acid + succinate + CO2
-
 ?
1.14.11.28 L-pipecolic acid + 2-oxoglutarate + O2
-
 Streptomyces sp. TH1 cis-3-hydroxy-L-pipecolic acid + succinate + CO2
-
 ?
1.14.11.28 L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. cis-3-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.28 L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. TH1 cis-3-hydroxy-L-proline + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.28 proline 3-hydroxylase type I
-
 Streptomyces sp.
1.14.11.28 proline 3-hydroxylase type II
-
 Streptomyces sp.