Literature summary extracted from
Pullen, K.E.; Ng, H.L.; Sung, P.Y.; Good, M.C.; Smith, S.M.; Alber, T.
An alternate conformation and a third metal in PstP/Ppp, the M. tuberculosis PP2C-Family Ser/Thr protein phosphatase (2004), Structure, 12, 1947-1954.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.3.16 |
expression in Escherichia coli strain BL21(DE3) as wild-type and selenomethionine-labeled variants |
Mycobacterium tuberculosis |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.1.3.16 |
purified recombinant wild-type and selenomethionine-labeled enzymes with bound phosphate, hanging drop vapour diffusion method, 18°C, 10 mg/ml protein in 50 mM NaCl, 20 mM Tris, pH 7.5, 0.5 mM Tris(2-carboxyethyl)phosphine hydrochloride, and 1 mM MnCl2, mixed with equal volume of reservoir solution containing 8% PEG 10000, 0.1 M sodium cacodylate, 0.5 mM TCEP, and 25% ethylene glycol, grown crystals are soaked in 0.1 M HEPES, pH 7.5, and 25 mM 4-nitrophenyl phosphate at 25°C, X-ray diffraction structure determination and analysis at 1.95 A resolution |
Mycobacterium tuberculosis |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.3.16 |
Mn2+ |
the enzyme contains a two-metal center |
Mycobacterium tuberculosis |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.3.16 |
Mycobacterium tuberculosis |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.3.16 |
recombinant wild-type and selenomethionine-labeled enzymes from Escherichia coli strain BL21(DE3) by gel filtration and ion exchange chromatography |
Mycobacterium tuberculosis |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
3.1.3.16 |
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate |
catalytic mechanism, PstPpp catalytic domain structure and active site structure, structure-function relationship |
Mycobacterium tuberculosis |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.3.16 |
4-nitrophenyl phosphate + H2O |
- |
Mycobacterium tuberculosis |
4-nitrophenol + phosphate |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.3.16 |
More |
the enzyme belongs to the Ser/Thr protein phosphatase PP2C-family |
Mycobacterium tuberculosis |
3.1.3.16 |
PstP/Ppp |
- |
Mycobacterium tuberculosis |
3.1.3.16 |
PstPpp |
- |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.1.3.16 |
25 |
- |
assay at |
Mycobacterium tuberculosis |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.1.3.16 |
7.5 |
- |
assay at |
Mycobacterium tuberculosis |