BRENDA - Enzyme Database

The role of isocitrate lyase and the glyoxylate cycle in Escherichia coli growing under glucose limitation

Prasad Maharjan, R.; Yu, P.L.; Seeto, S.; Ferenci, T.; Res. Microbiol. 156, 178-183 (2005)

Data extracted from this reference:

Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Escherichia coli
-
strains BW2952, BW3767, BW3709, BW3480, BW3708, BW3782, MG1655
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
culture condition:acetate-grown cell
0.2% acetate (batch)
Escherichia coli
-
4.1.3.1
culture condition:glucose-grown cell
0.02% glucose (chemostat); 0.2% glucose (batch)
Escherichia coli
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.1.3.1
0.004
-
strain BW3782, 0.02% glucose (chemostat); strain BW3782, 0.2% glucose (batch)
Escherichia coli
4.1.3.1
0.022
-
strain BW3709, 0.2% glucose (batch)
Escherichia coli
4.1.3.1
0.023
-
strain BW3767, 0.2% glucose (batch)
Escherichia coli
4.1.3.1
0.034
-
strain BW2952, 0.2% glucose (batch)
Escherichia coli
4.1.3.1
0.088
-
strain BW3480, 0.02% glucose (chemostat)
Escherichia coli
4.1.3.1
0.155
-
strain MG1655, 0.2% glucose (batch)
Escherichia coli
4.1.3.1
0.19
-
strain BW3708, 0.2% glucose (batch)
Escherichia coli
4.1.3.1
0.248
-
strain BW2952, 0.02% glucose (chemostat)
Escherichia coli
4.1.3.1
0.471
-
strain BW3767, 0.02% glucose (chemostat)
Escherichia coli
4.1.3.1
0.538
-
strain BW3709, 0.02% glucose (chemostat)
Escherichia coli
4.1.3.1
1.066
-
strain BW2952, 0.2% acetate (batch)
Escherichia coli
4.1.3.1
1.227
-
strain BW3767, 0.2% acetate (batch)
Escherichia coli
4.1.3.1
1.656
-
strain BW3709, 0.2% acetate (batch)
Escherichia coli
4.1.3.1
1.674
-
strain MG1655, 0.02% glucose (chemostat)
Escherichia coli
4.1.3.1
1.778
-
strain BW3708, 0.02% glucose (chemostat)
Escherichia coli
4.1.3.1
2.084
-
strain MG1655, 0.2% acetate (batch)
Escherichia coli
4.1.3.1
3.075
-
strain BW3708, 0.2% acetate (batch)
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
DL-isocitrate
-
666901
Escherichia coli
succinate + glyoxylate
-
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
culture condition:acetate-grown cell
0.2% acetate (batch)
Escherichia coli
-
4.1.3.1
culture condition:glucose-grown cell
0.02% glucose (chemostat); 0.2% glucose (batch)
Escherichia coli
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.1.3.1
0.004
-
strain BW3782, 0.02% glucose (chemostat); strain BW3782, 0.2% glucose (batch)
Escherichia coli
4.1.3.1
0.022
-
strain BW3709, 0.2% glucose (batch)
Escherichia coli
4.1.3.1
0.023
-
strain BW3767, 0.2% glucose (batch)
Escherichia coli
4.1.3.1
0.034
-
strain BW2952, 0.2% glucose (batch)
Escherichia coli
4.1.3.1
0.088
-
strain BW3480, 0.02% glucose (chemostat)
Escherichia coli
4.1.3.1
0.155
-
strain MG1655, 0.2% glucose (batch)
Escherichia coli
4.1.3.1
0.19
-
strain BW3708, 0.2% glucose (batch)
Escherichia coli
4.1.3.1
0.248
-
strain BW2952, 0.02% glucose (chemostat)
Escherichia coli
4.1.3.1
0.471
-
strain BW3767, 0.02% glucose (chemostat)
Escherichia coli
4.1.3.1
0.538
-
strain BW3709, 0.02% glucose (chemostat)
Escherichia coli
4.1.3.1
1.066
-
strain BW2952, 0.2% acetate (batch)
Escherichia coli
4.1.3.1
1.227
-
strain BW3767, 0.2% acetate (batch)
Escherichia coli
4.1.3.1
1.656
-
strain BW3709, 0.2% acetate (batch)
Escherichia coli
4.1.3.1
1.674
-
strain MG1655, 0.02% glucose (chemostat)
Escherichia coli
4.1.3.1
1.778
-
strain BW3708, 0.02% glucose (chemostat)
Escherichia coli
4.1.3.1
2.084
-
strain MG1655, 0.2% acetate (batch)
Escherichia coli
4.1.3.1
3.075
-
strain BW3708, 0.2% acetate (batch)
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
DL-isocitrate
-
666901
Escherichia coli
succinate + glyoxylate
-
-
-
?