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Literature summary extracted from

  • Romanello, V.; Marcacci, M.; Dal Molin, F.; Moschioni, M.; Censini, S.; Covacci, A.; Baritussio, A.G.; Montecucco, C.; Tonello, F.
    Cloning, expression, purification, and characterization of Streptococcus pneumoniae IgA1 protease (2006), Protein Expr. Purif., 45, 142-149.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.72 medicine the inactive mutant E1605A can be considered as a candidate vaccine component Streptococcus pneumoniae
3.4.24.13 medicine enzyme is a major Streptococcus pneumoniae antigen in humans Streptococcus pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.72 expression in Escherichia coli Streptococcus pneumoniae
3.4.24.13 three segments of enzyme, comprising amino acids 1032-1964, 708-1964, and 104-1964 Streptococcus pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.72 E1605A inactive Streptococcus pneumoniae
3.4.24.13 E1605A of segment 708-1964, completely inactive Streptococcus pneumoniae

General Stability

EC Number General Stability Organism
3.4.24.13 recombinant proteins are highly instable Streptococcus pneumoniae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.72 cell surface
-
 Streptococcus pneumoniae 9986
-
3.4.24.13 cell surface associated via an N-terminal membrane anchor, release upon proteolytic cleavage Streptococcus pneumoniae 9986
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.72 IgA1 + H2O Streptococcus pneumoniae
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.72 Streptococcus pneumoniae
-
-
-
3.4.24.13 Streptococcus pneumoniae Q59947
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.72
-
 Streptococcus pneumoniae
3.4.24.13 recombinant glutathione S-transferase fusion proteins of three segments of enzyme, comprising amino acids 1032-1964, 708-1964, and 104-1964. Segment 1032-1964 is catalytically inactive. Recombinant proteins are highly instable. Streptococcus pneumoniae

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.72 IgA1 + H2O
-
 Streptococcus pneumoniae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.72 IgA1 protease
-
 Streptococcus pneumoniae