Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Reilly, T.J.; Felts, R.L.; Henzl, M.T.; Calcutt, M.J.; Tanner, J.J.
    Characterization of recombinant Francisella tularensis acid phosphatase A (2006), Protein Expr. Purif., 45, 132-141.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2 gene acpA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strains DH5alpha, DH10B, and BL21(DE3), optimization of recombinant enzyme production Francisella tularensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 molybdate strong inhibition Francisella tularensis
3.1.3.2 tungstate strong inhibition Francisella tularensis
3.1.3.2 vanadate strong inhibition Francisella tularensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 additional information
-
-
 Francisella tularensis
3.1.3.2 57000
-
 x * 57000, recombinant enzyme, SDS-PAGE Francisella tularensis
3.1.3.2 136000
-
 analytical ultrafiltration and gel filtration Francisella tularensis

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Francisella tularensis
-
 human pathogen causing tularemia, gene acpA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 12.2fold of recombinant from Escherichia coli strain BL21(DE3) by cation exchange and nickel chelate affinity chromatography Francisella tularensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 0.0002
-
 purified recombinant enzyme Francisella tularensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 3'-GMP + H2O
-
 Francisella tularensis guanosine + phosphate
-
 ?
3.1.3.2 3'-TMP + H2O
-
 Francisella tularensis thymidine + phosphate
-
 ?
3.1.3.2 4-methylumbelliferyl phosphate + H2O
-
 Francisella tularensis 4-methylumbelliferol + phosphate
-
 ?
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Francisella tularensis 4-nitrophenol + phosphate
-
 ?
3.1.3.2 4-nitrophenyl phosphorylcholine + H2O at pH 7.4,synthetic substrate Francisella tularensis 4-nitrophenyl choline + phosphate
-
 ?
3.1.3.2 5'-GMP + H2O
-
 Francisella tularensis guanosine + phosphate
-
 ?
3.1.3.2 5'-UMP + H2O
-
 Francisella tularensis uridine + phosphate
-
 ?
3.1.3.2 ADP + H2O low activity Francisella tularensis AMP + phosphate
-
 ?
3.1.3.2 AMP + H2O
-
 Francisella tularensis adenosine + phosphate
-
 ?
3.1.3.2 ATP + H2O
-
 Francisella tularensis ADP + phosphate
-
 ?
3.1.3.2 D-fructose 1,6-bisphosphate + H2O
-
 Francisella tularensis ? + phosphate
-
 ?
3.1.3.2 additional information substrate specificity Francisella tularensis ?
-
 ?
3.1.3.2 NADP+ + H2O
-
 Francisella tularensis NAD+ + phosphate
-
 ?
3.1.3.2 phospho-L-threonine + H2O
-
 Francisella tularensis L-threonine + phosphate
-
 ?
3.1.3.2 phospho-L-tyrosine + H2O
-
 Francisella tularensis L-tyrosine + phosphate
-
 ?
3.1.3.2 phosphoenolpyruvate + H2O
-
 Francisella tularensis pyruvate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 oligomer x * 57000, recombinant enzyme, SDS-PAGE Francisella tularensis

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 acid phosphatase A
-
 Francisella tularensis
3.1.3.2 AcpA
-
 Francisella tularensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 37
-
 assay at Francisella tularensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 additional information
-
 the enzyme is heat-labile Francisella tularensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5.9 6.1
-
 Francisella tularensis