Literature summary extracted from
Li, H.; Zhang, X.
Characterization of thermostable lipase from thermophilic Geobacillus sp. TW1 (2005), Protein Expr. Purif., 42, 153-159.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.1.3 |
DNA and amino acid sequence determination and analysis, expression of GST-tagged enzyme in Escherichia coli |
Geobacillus stearothermophilus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.1.3 |
Cu2+ |
recombinant enzyme |
Geobacillus stearothermophilus |
|
3.1.1.3 |
Li+ |
recombinant enzyme |
Geobacillus stearothermophilus |
|
3.1.1.3 |
Mn2+ |
recombinant enzyme |
Geobacillus stearothermophilus |
|
3.1.1.3 |
additional information |
no inhibition by EDTA, PMSF, 2-mercaptoethanol, PMSF, DTT, Tween 20, 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonic acid, or Triton X-100 |
Geobacillus stearothermophilus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.1.3 |
Ca2+ |
activates, recombinant enzyme |
Geobacillus stearothermophilus |
|
3.1.1.3 |
Fe2+ |
activates, recombinant enzyme |
Geobacillus stearothermophilus |
|
3.1.1.3 |
Fe3+ |
activates, recombinant enzyme |
Geobacillus stearothermophilus |
|
3.1.1.3 |
Mg2+ |
activates, recombinant enzyme |
Geobacillus stearothermophilus |
|
3.1.1.3 |
Zn2+ |
activates, recombinant enzyme |
Geobacillus stearothermophilus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.1.3 |
Geobacillus stearothermophilus |
Q3T5M2 |
i.e. Bacillus stearothermophilus, strain TW1, isolated from a hot spring in China, gene lip2 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.1.3 |
recombinant GST-tagged enzyme from Escherichia coli |
Geobacillus stearothermophilus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.1.3 |
LIP2 |
- |
Geobacillus stearothermophilus |
3.1.1.3 |
lipase |
- |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.1.1.3 |
additional information |
- |
optimal growth temperature is 65°C |
Geobacillus stearothermophilus |
3.1.1.3 |
40 |
- |
recombinant enzyme |
Geobacillus stearothermophilus |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.1.1.3 |
90 |
- |
stable up to, purified recombinant enzyme |
Geobacillus stearothermophilus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.1.1.3 |
7 |
8 |
recombinant enzyme |
Geobacillus stearothermophilus |
pH Stability
EC Number |
pH Stability |
pH Stability Maximum |
Comment |
Organism |
---|
3.1.1.3 |
5 |
11 |
stable, purified recombinant enzyme |
Geobacillus stearothermophilus |