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Literature summary extracted from

  • Li, H.; Zhang, X.
    Characterization of thermostable lipase from thermophilic Geobacillus sp. TW1 (2005), Protein Expr. Purif., 42, 153-159.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 DNA and amino acid sequence determination and analysis, expression of GST-tagged enzyme in Escherichia coli Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Cu2+ recombinant enzyme Geobacillus stearothermophilus
3.1.1.3 Li+ recombinant enzyme Geobacillus stearothermophilus
3.1.1.3 Mn2+ recombinant enzyme Geobacillus stearothermophilus
3.1.1.3 additional information no inhibition by EDTA, PMSF, 2-mercaptoethanol, PMSF, DTT, Tween 20, 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonic acid, or Triton X-100 Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ activates, recombinant enzyme Geobacillus stearothermophilus
3.1.1.3 Fe2+ activates, recombinant enzyme Geobacillus stearothermophilus
3.1.1.3 Fe3+ activates, recombinant enzyme Geobacillus stearothermophilus
3.1.1.3 Mg2+ activates, recombinant enzyme Geobacillus stearothermophilus
3.1.1.3 Zn2+ activates, recombinant enzyme Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Geobacillus stearothermophilus Q3T5M2 i.e. Bacillus stearothermophilus, strain TW1, isolated from a hot spring in China, gene lip2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 recombinant GST-tagged enzyme from Escherichia coli Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 LIP2
-
 Geobacillus stearothermophilus
3.1.1.3 lipase
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 additional information
-
 optimal growth temperature is 65°C Geobacillus stearothermophilus
3.1.1.3 40
-
 recombinant enzyme Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 90
-
 stable up to, purified recombinant enzyme Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 7 8 recombinant enzyme Geobacillus stearothermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 5 11 stable, purified recombinant enzyme Geobacillus stearothermophilus