EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.1 | Ag+ | - |
Bacillus subtilis | |
3.2.1.1 | Cu2+ | - |
Bacillus subtilis | |
3.2.1.1 | Hg2+ | - |
Bacillus subtilis | |
3.2.1.1 | additional information | mo inhibition by EDTA or EGTA | Bacillus subtilis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.1 | extracellular | - |
Bacillus subtilis | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.1 | additional information | the enzyme shows no requirement for metals | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 79000 | - |
2 * 79000, SDS-PAGE | Bacillus subtilis |
3.2.1.1 | 139400 | - |
gel filtration | Bacillus subtilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.1 | starch + H2O | Bacillus subtilis | - |
maltose + D-glucose | - |
? | |
3.2.1.1 | starch + H2O | Bacillus subtilis AX20 | - |
maltose + D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.1 | Bacillus subtilis | - |
isolated from soil | - |
3.2.1.1 | Bacillus subtilis AX20 | - |
isolated from soil | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.1 | glycoprotein | - |
Bacillus subtilis |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.1 | native extracellular enzyme 37.5fold to homogeneity by ammonium sulfate fractionation, ion exchange and starch affinity chromatography, and gel filtration | Bacillus subtilis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.2.1.1 | (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-m-alpha-D-glucopyranose + (alpha-D-glucopyranosyl-(1-4))m-alpha-D-glucopyranose | endo mode of action | Bacillus subtilis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 4133 | - |
purified enzyme | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.1 | starch + H2O | - |
Bacillus subtilis | maltose + D-glucose | - |
? | |
3.2.1.1 | starch + H2O | raw starch from corn and soluble starch, endo mode of action | Bacillus subtilis | maltose + D-glucose | 25% maltose, 75% glucose | ? | |
3.2.1.1 | starch + H2O | - |
Bacillus subtilis AX20 | maltose + D-glucose | - |
? | |
3.2.1.1 | starch + H2O | raw starch from corn and soluble starch, endo mode of action | Bacillus subtilis AX20 | maltose + D-glucose | 25% maltose, 75% glucose | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.1 | dimer | 2 * 79000, SDS-PAGE | Bacillus subtilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 55 | - |
- |
Bacillus subtilis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 50 | - |
30 min, purified enzyme, stable | Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 6 | - |
- |
Bacillus subtilis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 5 | 9 | purified enzyme, stable, 24 h at 4°C | Bacillus subtilis |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.1 | Bacillus subtilis | isoelectric focusing | - |
7 |