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Literature summary extracted from

  • Cieslinski, H.; Kur, J.; Bialkowska, A.; Baran, I.; Makowski, K.; Turkiewicz, M.
    Cloning, expression, and purification of a recombinant cold-adapted beta-galactosidase from antarctic bacterium Pseudoalteromonas sp. 22b (2005), Protein Expr. Purif., 39, 27-34.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.23 nutrition recombinant Pseudoalteromonas sp. 22b beta-galactosidase could be applied at refrigeration temperatures for production of lactose-reduced dairy products Pseudoalteromonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.23 expression in Escherichia coli Pseudoalteromonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Pseudoalteromonas sp. Q6RGS1
-
-
3.2.1.23 Pseudoalteromonas sp. 22b Q6RGS1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Pseudoalteromonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 lactose + H2O 90% of milk lactose is hydrolyzed during 6 h at 30°C and during 28 h at 15°C Pseudoalteromonas sp. D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O 90% of milk lactose is hydrolyzed during 6 h at 30°C and during 28 h at 15°C Pseudoalteromonas sp. 22b D-glucose + D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Pseudoalteromonas sp. o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Pseudoalteromonas sp. 22b o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O 58% higher activity than with o-nitrophenyl-beta-D-galactoside Pseudoalteromonas sp. p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O 58% higher activity than with o-nitrophenyl-beta-D-galactoside Pseudoalteromonas sp. 22b p-nitrophenol + beta-D-galactose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 40
-
-
 Pseudoalteromonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.23 10 40 10°C: 20% of maximal activity, 40°C: optimum Pseudoalteromonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 50
-
 10 min, complete loss of activity Pseudoalteromonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 6 8
-
 Pseudoalteromonas sp.