Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, B.; Lu, D.; Gao, R.; Yang, Z.; Cao, S.; Feng, Y.
    A novel phospholipase A2/esterase from hyperthermophilic archaeon Aeropyrum pernix K1 (2004), Protein Expr. Purif., 35, 199-205.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.4 expression in Escherichia coli Aeropyrum pernix

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 CaCl2 1 mM, residual activity 82.1% Aeropyrum pernix
3.1.1.4 KCl 1 mM, residual activity 78.9% Aeropyrum pernix
3.1.1.4 MgCl2 1 mM, residual activity 71.6% Aeropyrum pernix
3.1.1.4 additional information not inhibitory: EDTA Aeropyrum pernix
3.1.1.4 NaCl 1 mM, residual activity 58.5% Aeropyrum pernix
3.1.1.4 ZnCl2 1 mM, no residual activity Aeropyrum pernix

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.4 0.103
-
 p-nitrophenyl propionate 90°C, pH 8.0 Aeropyrum pernix

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 additional information no requirement for Ca2+ Aeropyrum pernix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 18000
-
 x * 18000, SDS-PAGE Aeropyrum pernix

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Aeropyrum pernix
-
 var. K1, bifunctional enzyme bwith both phospholipase A2 and esterase activity
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 249
-
 90°C, pH 8.0 Aeropyrum pernix

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 additional information no substrate: p-nitrophenyl palmitate, p-nitrophenyl stearate Aeropyrum pernix ?
-
 ?
3.1.1.4 p-nitrophenyl laurate + H2O 23.7% of the activity with p-nitrophenyl propionate Aeropyrum pernix p-nitrophenol + laurate
-
 ?
3.1.1.4 p-nitrophenyl octanoate + H2O 79.5% of the activity with p-nitrophenyl propionate Aeropyrum pernix p-nitrophenol + octanoic acid
-
 ?
3.1.1.4 p-nitrophenyl propionate + H2O
-
 Aeropyrum pernix p-nitrophenol + propionate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.4 ? x * 18000, SDS-PAGE Aeropyrum pernix

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.4 90
-
-
 Aeropyrum pernix

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.4 100
-
 half-life of about 1 h Aeropyrum pernix

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.4 39
-
 p-nitrophenyl propionate 90°C, pH 8.0 Aeropyrum pernix