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Literature summary extracted from
- Structural and biochemical studies of GH family 12 cellulases: improved thermal stability, and ligand complexes (2005), Prog. Biophys. Mol. Biol., 89, 246-291.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.4 | exprression in Aspergillus niger | Trichoderma reesei |
| 3.2.1.4 | exprression in Aspergillus niger | Trichoderma citrinoviride |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.2.1.4 | - |
Trichoderma reesei |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | G170C | Tm is 2.1°C higher than wild-type value, specific activity is 1.2fold higher than wild-type enzyme | Trichoderma reesei |
| 3.2.1.4 | G170C/P201C | Tm is 0.7°C higher than wild-type value, specific activity is 38% of wild-type value | Trichoderma reesei |
| 3.2.1.4 | G170C/P201C/V210C | Tm is 3.9°C higher than wild-type value, specific activity is 14% of wild-type value | Trichoderma reesei |
| 3.2.1.4 | G170C/V210C | specific activity is 1.4fold higher than wild-type value | Trichoderma reesei |
| 3.2.1.4 | P201C | Tm is 0.7°C higher than wild-type value, specific activity is 50% of wild-type value | Trichoderma reesei |
| 3.2.1.4 | P201C | Tm is 3.9°C higher than wild-type value, specific activity is 80% of wild-type value | Trichoderma reesei |
| 3.2.1.4 | V210C | Tm is 0.1°C higher than wild-type value, specific activity is 1.8fold of the wild-type value | Trichoderma reesei |
| 3.2.1.4 | V210C | Tm is identical to wild-type value, specific activity is 80% of wild-type value | Trichoderma reesei |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 25000 | - |
- |
Trichoderma reesei |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.4 | Clonostachys rosea | - |
- |
- |
| 3.2.1.4 | Fusarium solani-melongenae | - |
- |
- |
| 3.2.1.4 | Trichocladium griseum | - |
- |
- |
| 3.2.1.4 | Trichoderma citrinoviride | - |
- |
- |
| 3.2.1.4 | Trichoderma koningii | - |
- |
- |
| 3.2.1.4 | Trichoderma reesei | O00095 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | glycoprotein | sparsely glycosylated | Trichoderma reesei |
| 3.2.1.4 | additional information | the N-terminal glutamine undergoes a cyclization and condensation reaction with the amino group of the N-terminus, to produce a cyclic diglutamate | Trichoderma reesei |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.4 | cellulose + H2O | - |
Clonostachys rosea | ? | - |
? |  |
| 3.2.1.4 | cellulose + H2O | - |
Trichoderma koningii | ? | - |
? | |
| 3.2.1.4 | cellulose + H2O | - |
Trichocladium griseum | ? | - |
? | |
| 3.2.1.4 | cellulose + H2O | - |
Fusarium solani-melongenae | ? | - |
? | |
| 3.2.1.4 | cellulose + H2O | - |
Trichoderma reesei | ? | - |
? | |
| 3.2.1.4 | cellulose + H2O | - |
Trichoderma citrinoviride | ? | - |
? | |
| 3.2.1.4 | o-nitrophenyl beta-cellobioside + H2O | - |
Clonostachys rosea | ? | - |
? | |
| 3.2.1.4 | o-nitrophenyl beta-cellobioside + H2O | - |
Trichoderma koningii | ? | - |
? | |
| 3.2.1.4 | o-nitrophenyl beta-cellobioside + H2O | - |
Trichocladium griseum | ? | - |
? | |
| 3.2.1.4 | o-nitrophenyl beta-cellobioside + H2O | - |
Fusarium solani-melongenae | ? | - |
? | |
| 3.2.1.4 | o-nitrophenyl beta-cellobioside + H2O | - |
Trichoderma reesei | ? | - |
? | |
| 3.2.1.4 | o-nitrophenyl beta-cellobioside + H2O | - |
Trichoderma citrinoviride | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | Cel12A | - |
Clonostachys rosea |
| 3.2.1.4 | Cel12A | - |
Trichoderma koningii |
| 3.2.1.4 | Cel12A | - |
Trichocladium griseum |
| 3.2.1.4 | Cel12A | - |
Fusarium solani-melongenae |
| 3.2.1.4 | Cel12A | - |
Trichoderma reesei |
| 3.2.1.4 | Cel12A | - |
Trichoderma citrinoviride |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 46 | - |
Tm: 45.9°C | Clonostachys rosea |
| 3.2.1.4 | 49 | - |
Tm: 49.2°C | Trichoderma citrinoviride |
| 3.2.1.4 | 54 | - |
Tm: 54.1°C | Fusarium solani-melongenae |
| 3.2.1.4 | 54 | - |
Tm: 54.4°C | Trichoderma reesei |
| 3.2.1.4 | 58.5 | - |
Tm-value | Trichoderma koningii |
| 3.2.1.4 | 67 | - |
Tm: 66.8°C | Trichoderma citrinoviride |
| 3.2.1.4 | 69 | - |
Tm: 68.6°C | Trichocladium griseum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 5.5 | - |
- |
Trichoderma koningii |
| 3.2.1.4 | 5.5 | - |
- |
Trichocladium griseum |
| 3.2.1.4 | 5.5 | - |
- |
Trichoderma citrinoviride |
| 3.2.1.4 | 5.5 | - |
substrate: o-nitrophenyl beta-cellobioside | Trichoderma reesei |
| 3.2.1.4 | 8 | - |
- |
Trichoderma citrinoviride |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 5 | - |
optimal stability | Clonostachys rosea |
| 3.2.1.4 | 5 | - |
optimal stability | Trichoderma koningii |
| 3.2.1.4 | 5 | - |
optimal stability | Fusarium solani-melongenae |
| 3.2.1.4 | 5 | - |
optimal stability | Trichoderma reesei |
| 3.2.1.4 | 5 | - |
optimal stability | Trichoderma citrinoviride |
| 3.2.1.4 | 7 | - |
optimal stability | Trichocladium griseum |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.4 | Trichoderma reesei | - |
- |
7.5 |
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Release 2023.1 (January 2023)
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