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Literature summary extracted from

  • Bernhardsdotter, E.C.; Ng, J.D.; Garriott, O.K.; Pusey, M.L.
    Enzymic properties of an alkaline chelator-resistant alpha-amylase from an alkaliphilic Bacillus sp. isolate L1711 (2005), Process Biochem., 40, 2401-2408.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.1 EDTA activation of isozyme BAA at 5-10 mM Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 Ba2+ strong inhibition of isozyme BAA Bacillus sp. (in: Bacteria)
3.2.1.1 Ca2+ strong inhibition of isozyme BAA Bacillus sp. (in: Bacteria)
3.2.1.1 Cu2+ strong inhibition of isozyme BAA Bacillus sp. (in: Bacteria)
3.2.1.1 Mg2+ strong inhibition of isozyme BAA Bacillus sp. (in: Bacteria)
3.2.1.1 Mn2+ strong inhibition of isozyme BAA Bacillus sp. (in: Bacteria)
3.2.1.1 PMSF
-
 Bacillus sp. (in: Bacteria)
3.2.1.1 Zn2+ strong inhibition of isozyme BAA Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 1.9
-
 soluble starch pH 10.0, 40°C Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 Co2+ activation of isozyme BAA at 10 mM Bacillus sp. (in: Bacteria)
3.2.1.1 Na+ activation of isozyme BAA at 10 mM Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 51000
-
 x * 51000, isozyme BAA, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Bacillus sp. (in: Bacteria)
-
 isolate L1711, several isozymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 native extracellular isozyme BAA from culture medium by ion exchange chromatographyand preparative PAGE Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 18.5
-
 purified isozyme BAA Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 starch + H2O soluble starch Bacillus sp. (in: Bacteria) malto-oligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ? x * 51000, isozyme BAA, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 BAA
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 40
-
 isozyme BAA Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 4
-
 1 h, purified isozyme BAA, completely stable Bacillus sp. (in: Bacteria)
3.2.1.1 35
-
 1 h, purified isozyme BAA, 84.3% remaining activity Bacillus sp. (in: Bacteria)
3.2.1.1 55
-
 1 h, purified isozyme BAA, inactivation in presence of Ca2+, 1.6% residual activity in absence of Ca2+ Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 7 10 depends strongly on the temperature Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.1 7 10
-
 Bacillus sp. (in: Bacteria)