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Literature summary extracted from

  • Zhang, C.; Zhang, Y.; Chen, J.; Liang, X.
    Purification and characterization of baicalin-b-D-glucuronidase hydrolyzing baicalin to baicalein from fresh roots of Scutellaria viscidula Bge (2005), Process Biochem., 40, 1911-1915.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.167 Cu2+ inhibits 29% at 100 mM Scutellaria viscidula
3.2.1.167 Fe2+ inhibits 29% at 1 mM, 71% at 100 mM Scutellaria viscidula
3.2.1.167 Zn2+ inhibits 7% at 100 mM Scutellaria viscidula

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.167 Ca2+ activates 7% at 100 mM Scutellaria viscidula
3.2.1.167 Mg2+ activates 26% at 100 mM Scutellaria viscidula

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.167 58400
-
 x * 58400, SDS-PAGE Scutellaria viscidula

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.167 baicalin + H2O Scutellaria viscidula
-
 baicalein + beta-D-glucuronic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.167 Scutellaria viscidula
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.167 native enzyme 7.2fold from roots, by ammonium sulfate fractionation and anion exchange chromatography to homogeneity Scutellaria viscidula

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.167 root fresh Scutellaria viscidula
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.167 additional information
-
-
 Scutellaria viscidula
3.2.1.167 9.37
-
 purified enzyme, substrate 4-nitrophenyl beta-D-glucuronide Scutellaria viscidula

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.167 4-nitrophenyl beta-D-glucuronide + H2O
-
 Scutellaria viscidula 4-nitrophenol + beta-D-glucuronic acid
-
 ?
3.2.1.167 4-phenolphthaleinyl beta-D-glucuronide + H2O
-
 Scutellaria viscidula 4-phenolphthalein + beta-D-glucuronic acid
-
 ?
3.2.1.167 baicalin + H2O
-
 Scutellaria viscidula baicalein + beta-D-glucuronic acid
-
 ?
3.2.1.167 baicalin + H2O best substrate Scutellaria viscidula baicalein + beta-D-glucuronic acid
-
 ?
3.2.1.167 additional information no activity with 4-nitrophenyl beta-D-glucoside Scutellaria viscidula ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.167 ? x * 58400, SDS-PAGE Scutellaria viscidula

Synonyms

EC Number Synonyms Comment Organism
3.2.1.167 baicalin-beta-D-glucuronidase
-
 Scutellaria viscidula

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.167 50
-
-
 Scutellaria viscidula

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.167 20 70
-
 Scutellaria viscidula

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.167 70
-
 30 min, stable up to Scutellaria viscidula
3.2.1.167 80
-
 inactivation Scutellaria viscidula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.167 5
-
-
 Scutellaria viscidula

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.167 3 7
-
 Scutellaria viscidula