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Literature summary extracted from

  • Dong, Y.H.; Xu, J.L.; Li, X.Z.; Zhang, L.H.
    AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora (2000), Proc. Natl. Acad. Sci. USA, 97, 3526-3531.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.81 gene aiiA, DNA and amino acid sequence determination and analysis, expression of GST-tagged AiiA in Escherichia coli strain DH5alpha, expression in Erwinia carotovora strain SCG1 leading to reduction of autoinducer release thereby cedreasing extracellular pectolytic enzyme activities, and attenuating pathogenicity on potato, eggplant, Chinese cabbage, carrot, celery, cauliflower, and tobacco Bacillus sp. (in: Bacteria)

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.81 D108E site-directed mutagenesis, mutation of a zinc-binding motif residue, the mutant shows 90.8% of wild-type enzyme activity Bacillus sp. (in: Bacteria)
3.1.1.81 D108S site-directed mutagenesis, mutation of a zinc-binding motif residue, inactive mutant Bacillus sp. (in: Bacteria)
3.1.1.81 D108S/H109S site-directed mutagenesis, mutation of zinc-binding motif residues, inactive mutant Bacillus sp. (in: Bacteria)
3.1.1.81 H104L/H106L/D108L site-directed mutagenesis, mutation of zinc-binding motif residues, the mutant shows 61.4% of wild-type enzyme activity Bacillus sp. (in: Bacteria)
3.1.1.81 H104L/H106L/D108L/H109L site-directed mutagenesis, mutation of zinc-binding motif residues, the mutant shows 37.9% of wild-type enzyme activity Bacillus sp. (in: Bacteria)
3.1.1.81 H104S site-directed mutagenesis, mutation of a zinc-binding motif residue, the mutant is as active as the wild-type enzyme Bacillus sp. (in: Bacteria)
3.1.1.81 H104S/H106S site-directed mutagenesis, mutation of zinc-binding motif residues, the mutant shows 51.1% of wild-type enzyme activity Bacillus sp. (in: Bacteria)
3.1.1.81 H104S/H106S/D108S/H109Sc site-directed mutagenesis, mutation of zinc-binding motif residues, inactive mutant Bacillus sp. (in: Bacteria)
3.1.1.81 H104S/H106S/H109S site-directed mutagenesis, mutation of zinc-binding motif residues, inactive mutant Bacillus sp. (in: Bacteria)
3.1.1.81 H106S site-directed mutagenesis, mutation of a zinc-binding motif residue, the mutant shows 61.4% of wild-type enzyme activity Bacillus sp. (in: Bacteria)
3.1.1.81 H109S site-directed mutagenesis, mutation of a zinc-binding motif residue, inactive mutant Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.81 Zn2+ metallohydrolase containing the conserved HXHXDH zinc-binding motif required for full enzyme activity Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.81 N-acyl-(S)-homoserine lactone + H2O Bacillus sp. (in: Bacteria) the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, mechanism N-acyl-(S)-homoserine
-
 ?
3.1.1.81 N-acyl-(S)-homoserine lactone + H2O Bacillus sp. (in: Bacteria) 240B1 the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, mechanism N-acyl-(S)-homoserine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.81 Bacillus sp. (in: Bacteria) Q9L8R8 isolated from soil, gene aiiA
-
3.1.1.81 Bacillus sp. (in: Bacteria) 240B1 Q9L8R8 isolated from soil, gene aiiA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.81 recombinant GST-tagged AiiA from Escherichia coli strain DH5alpha Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.81 N-3-oxodecanoyl-L-homoserine lactone + H2O
-
 Bacillus sp. (in: Bacteria) N-3-oxodecanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxodecanoyl-L-homoserine lactone + H2O
-
 Bacillus sp. (in: Bacteria) 240B1 N-3-oxodecanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Bacillus sp. (in: Bacteria) N-3-oxohexanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Bacillus sp. (in: Bacteria) 240B1 N-3-oxohexanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxooctanoyl-L-homoserine lactone + H2O
-
 Bacillus sp. (in: Bacteria) N-3-oxooctanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxooctanoyl-L-homoserine lactone + H2O
-
 Bacillus sp. (in: Bacteria) 240B1 N-3-oxooctanoyl-L-homoserine
-
 ?
3.1.1.81 N-acyl-(S)-homoserine lactone + H2O the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, mechanism Bacillus sp. (in: Bacteria) N-acyl-(S)-homoserine
-
 ?
3.1.1.81 N-acyl-(S)-homoserine lactone + H2O the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, mechanism Bacillus sp. (in: Bacteria) 240B1 N-acyl-(S)-homoserine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.81 AiiA
-
 Bacillus sp. (in: Bacteria)