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Literature summary extracted from

  • Arita, K.; Shimizu, T.; Hashimoto, H.; Hidaka, Y.; Yamada, M.; Sato, M.
    Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4 (2006), Proc. Natl. Acad. Sci. USA, 103, 5291-5296.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.15 overexpression of GST-tagged PAD4 mutant C645A Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.3.15 crystal structures of a Ca2+-bound PAD4 mutant C645A in complex with three histone N-terminal peptides, each consisting of 10 amino acid residues that include one target arginine residue for the enzyme (H3/Arg-8, H3/Arg-17, and H4/Arg-3). hanging-drop vapor-diffusion method Homo sapiens
3.5.3.15 purified recombinant GST-tagged PAD4 mutant C645A in complex with Ca2+ and histone-derived N-terminal peptide substrates, hanging-drop vapor-diffusion method, soaking of Ca2+-free crystals in crystallization buffer containing 5 mM CaCl2 and each histone N-terminal peptide for 8 h at 20°C, X-ray diffraction structure determination and anaylsis at 2.0-2.225 A resolution, molecular replacement Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.3.15 C645A inactive mutant Homo sapiens
3.5.3.15 C645A site-directed mutagemesis, inactive mutant, substrate-bound structure analysis Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.3.15 nucleus PAD4 contains nuclear localization signal region in the subdomain 1 Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.15 Ca2+ dependent on, binding structure, overview Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.15 histone H3-L-arginine + H2O Homo sapiens
-
 histone H3-L-citrulline + NH3
-
 ?
3.5.3.15 protein-L-arginine + H2O Homo sapiens
-
 protein-L-citrulline + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.15 Homo sapiens
-
-
-
3.5.3.15 Homo sapiens Q9UM07 PAD4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.15 recombinant GST-tagged PAD4 mutant C645A by glutathione affinity chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.15 histone H3-L-arginine + H2O
-
 Homo sapiens histone H3-L-citrulline + NH3
-
 ?
3.5.3.15 additional information substrate binding structure, sequence specificity, overview Homo sapiens ?
-
 ?
3.5.3.15 peptide H3-1-L-arginine + H2O i.e. Ac4KQTARKSTGG13 Homo sapiens peptide H3-1-L-citrulline + NH3
-
 ?
3.5.3.15 peptide H3-2-L-arginine + H2O i.e. Ac14KAPRKQLATK23 Homo sapiens peptide H3-2-L-citrulline + NH3
-
 ?
3.5.3.15 peptide H4-L-arginine + H2O
-
 Homo sapiens peptide H4-L-citrulline + NH3 i.e. Ac1SGRGKGGKGL10 ?
3.5.3.15 protein-L-arginine + H2O
-
 Homo sapiens protein-L-citrulline + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.3.15 PAD4
-
 Homo sapiens
3.5.3.15 peptidylarginine deiminase 4
-
 Homo sapiens