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Literature summary extracted from

  • Lerner-Marmarosh, N.; Shen, J.; Torno, M.D.; Kravets, A.; Hu, Z.; Maines, M.D.
    Human biliverdin reductase: A member of the insulin receptor substrate family with serine/threonine/tyrosine kinase activity (2005), Proc. Natl. Acad. Sci. USA, 102, 7109-7114.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.24 Insulin increases BVR tyrosine autophosphorylation Homo sapiens

Application

EC Number Application Comment Organism
1.3.1.24 additional information potential role in the insulin signaling pathway, BVR is both a substrate for insulin receptor tyrosine kinase activity and a kinase for serine phosphorylation of insulin receptor substrate 1 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.24 into vector pcDNA3, transformed into Escherichia coli DH5alpha, pGEX-4T2/human BVR vector Homo sapiens
2.7.12.1 expression in Escherichia coli strain DH5alpha as GST-fusion protein, expression in 293A cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.24 additional information point mutations of serine residues in the kinase domain of the enzyme inhibits phosphotransferase activity Homo sapiens
2.7.12.1 additional information enzyme knock-out leads to increased D-glucose uptake in response to insulin Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.24 Ca2+ abolishes autophosphorylation of BVR Homo sapiens
1.3.1.24 Mg2+ abolishes autophosphorylation of BVR Homo sapiens
1.3.1.24 Zn2+ inhibitory to autophosphorylation of BVR, can not be overcome with Mn2+ Homo sapiens
2.7.12.1 genistein
-
 Homo sapiens
2.7.12.1 Zn2+ inhibits the autophosphoylation, which cannot be reversed by Mn2+ addition Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.24 Mn2+ acitvates BVR tyrosine kinase activity Homo sapiens
2.7.12.1 Mg2+ required for kinase activity Homo sapiens
2.7.12.1 Mn2+ essential for autophosphorylation activity, cannot be substituted by Mg2+, Ca2+, or Zn2+ Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.12.1 ATP + insulin receptor kinase substrate 1 Homo sapiens phosphorylation at serine residues, overview, the enzyme is involved in the insulin signaling pathway ADP + phosphorylated insulin receptor kinase substrate 1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.24 Homo sapiens
-
-
-
2.7.12.1 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.12.1 phosphoprotein the enzyme autophosphorylates at Thr202, Tyr72 and Tyr83, the insulin-activated insulin receptor kinase phosphorylates Y198 in the YMKM motif, Y228 in the YLSF motif, and Y291 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.12.1 recombinant GST-fusion protein from Escherichia coli strain DH5alpha by glutathione affinity chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.24 insulin receptor substrate-1 + NAD(P)H the serine residues are targets for BVR phosphorylation Homo sapiens ?
-
 ?
2.7.12.1 ATP + casein
-
 Homo sapiens ADP + phosphorylated casein
-
 ?
2.7.12.1 ATP + insulin receptor kinase substrate 1 phosphorylation at serine residues, overview, the enzyme is involved in the insulin signaling pathway Homo sapiens ADP + phosphorylated insulin receptor kinase substrate 1
-
 ?
2.7.12.1 ATP + insulin receptor kinase substrate 1 phosphorylation at serine residues leading to blockage of insulin action, overview Homo sapiens ADP + phosphorylated insulin receptor kinase substrate 1
-
 ?
2.7.12.1 ATP + myelin basic protein
-
 Homo sapiens ADP + phosphorylated myelin basic protein
-
 ?
2.7.12.1 additional information the enzyme performs autophosphorylation at Thr202, Tyr72 and Tyr83, the enzyme also shows biliverdin reductase activity Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.24 BVR
-
 Homo sapiens
2.7.12.1 biliverdin reductase
-
 Homo sapiens
2.7.12.1 BVR
-
 Homo sapiens
2.7.12.1 More the enzyme belongs to the insulin receptor substrate family Homo sapiens
2.7.12.1 serine/threonine/tyrosine kinase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.12.1 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.12.1 8.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.12.1 ATP
-
 Homo sapiens