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Literature summary extracted from

  • Aharoni, A.; Gaidukov, L.; Yagur, S.; Toker, L.; Silman, I.; Tawfik, D.S.
    Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization (2004), Proc. Natl. Acad. Sci. USA, 101, 482-487.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.2 application of directed evolution in achieving functional expression of PON1 and PON3 in Escherichia coli culture and a dramatic increase in their hydrolytic proficiency toward the fluorogenic organophosphate substrate 7-O-diethylphosphoryl-3-cyano-7-hydroxycoumarin. The powerful tool of directed evolution opens new prospects for improving their phosphotriesterase activity toward other hazardous organophosphates and toward catalytic activities related to the prevention of atherosclerosis Homo sapiens
3.1.8.1 application of directed evolution in achieving functional expression of PON1 and PON3 in Escherichia coli culture and a dramatic increase in their hydrolytic proficiency toward the fluorogenic organophosphate substrate 7-O-diethylphosphoryl-3-cyano-7-hydroxycoumarin. The powerful tool of directed evolution opens new prospects for improving their phosphotriesterase activity toward other hazardous organophosphates and toward catalytic activities related to the prevention of atherosclerosis Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.2 0.16
-
2-naphthyl acetate pH 8.0, recombinant genetic variant G1A7 Homo sapiens
3.1.1.2 0.18
-
2-naphthyl acetate pH 8.0, recombinant genetic variant G1B11 Homo sapiens
3.1.1.2 0.211
-
2-naphthyl acetate pH 8.0 Oryctolagus cuniculus
3.1.1.2 0.26
-
2-naphthyl acetate pH 8.0, recombinant genetic variant G2C2 Homo sapiens
3.1.1.2 0.5
-
2-naphthyl acetate pH 8.0 Mus musculus
3.1.1.2 0.65
-
2-naphthyl acetate pH 8.0, recombinant genetic variant G3G3 Homo sapiens
3.1.8.1 0.085
-
paraoxon pH 8.0, recombinant genetic variant G1A5 Homo sapiens
3.1.8.1 0.088
-
paraoxon pH 8.0, recombinant genetic variant G3H8 Homo sapiens
3.1.8.1 0.089
-
paraoxon pH 8.0, recombinant genetic variant G2E6 Homo sapiens
3.1.8.1 0.094
-
paraoxon pH 8.0, recombinant genetic variant G3C9 Homo sapiens
3.1.8.1 0.1
-
paraoxon pH 8.0, recombinant genetic variant G2D6 Homo sapiens
3.1.8.1 0.12
-
paraoxon pH 8.0, recombinant genetic variant G1C4 Homo sapiens
3.1.8.1 0.51
-
paraoxon pH 8.0, recombinant genetic variant G3A5 Homo sapiens
3.1.8.1 0.54
-
paraoxon pH 8.0, wild-type enzyme Homo sapiens
3.1.8.1 0.75
-
paraoxon pH 8.0, recombinant genetic variant G3G3 Homo sapiens
3.1.8.1 0.8
-
paraoxon pH 8.0, recombinant genetic variant G2C2 Homo sapiens
3.1.8.1 0.8
-
paraoxon pH 8.0, recombinant genetic variant G3H9 Homo sapiens
3.1.8.1 1.1
-
paraoxon pH 8.0, recombinant genetic variant G1B11 Homo sapiens
3.1.8.1 1.3
-
paraoxon pH 8.0 Oryctolagus cuniculus
3.1.8.1 1.4
-
paraoxon pH 8.0 Mus musculus
3.1.8.1 2.5
-
paraoxon pH 8.0, recombinant genetic variant G1A7 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.2 Homo sapiens Q15166
-
-
3.1.1.2 Mus musculus Q62087
-
-
3.1.1.2 Oryctolagus cuniculus Q9BGN0
-
-
3.1.8.1 Homo sapiens P27169
-
-
3.1.8.1 Homo sapiens Q15166
-
-
3.1.8.1 Mus musculus Q62087
-
-
3.1.8.1 Oryctolagus cuniculus Q9BGN0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.2
-
Homo sapiens
3.1.8.1
-
Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.2 2-naphthyl acetate + H2O
-
Homo sapiens 2-naphthol + acetate
-
?
3.1.1.2 2-naphthyl acetate + H2O
-
Oryctolagus cuniculus 2-naphthol + acetate
-
?
3.1.1.2 2-naphthyl acetate + H2O
-
Mus musculus 2-naphthol + acetate
-
?
3.1.8.1 7-O-diethylphosphoryl-3-cyano-7-hydroxycoumarin + H2O
-
Homo sapiens ?
-
?
3.1.8.1 paraoxon + H2O
-
Homo sapiens diethylphosphate + 4-nitrophenol
-
?
3.1.8.1 paraoxon + H2O
-
Homo sapiens 4-nitrophenol + diethyl phosphate
-
?
3.1.8.1 paraoxon + H2O
-
Oryctolagus cuniculus 4-nitrophenol + diethyl phosphate
-
?
3.1.8.1 paraoxon + H2O
-
Mus musculus 4-nitrophenol + diethyl phosphate
-
?
3.1.8.1 phenyl acetate + H2O
-
Homo sapiens phenol + acetate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.8.1 PON1
-
Homo sapiens
3.1.8.1 PON3
-
Homo sapiens
3.1.8.1 PON3
-
Oryctolagus cuniculus
3.1.8.1 PON3
-
Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.2 0.66
-
2-naphthyl acetate pH 8.0 Oryctolagus cuniculus
3.1.1.2 1.9
-
2-naphthyl acetate pH 8.0, recombinant genetic variant G1A7 Homo sapiens
3.1.1.2 2
-
2-naphthyl acetate pH 8.0, recombinant genetic variant G1B11 Homo sapiens
3.1.1.2 2.12
-
2-naphthyl acetate pH 8.0 Mus musculus
3.1.1.2 18.8
-
2-naphthyl acetate pH 8.0, recombinant genetic variant G2C2 Homo sapiens
3.1.1.2 26.25
-
2-naphthyl acetate pH 8.0, recombinant genetic variant G3G3 Homo sapiens
3.1.8.1 0.0008
-
paraoxon pH 8.0 Mus musculus
3.1.8.1 0.001
-
paraoxon pH 8.0 Oryctolagus cuniculus
3.1.8.1 0.007
-
paraoxon pH 8.0, recombinant genetic variant G1A7 Homo sapiens
3.1.8.1 0.009
-
paraoxon pH 8.0, recombinant genetic variant G1B11 Homo sapiens
3.1.8.1 0.036
-
paraoxon pH 8.0, recombinant genetic variant G2C2 Homo sapiens
3.1.8.1 0.04
-
paraoxon pH 8.0, recombinant genetic variant G3A5 Homo sapiens
3.1.8.1 0.11
-
paraoxon pH 8.0, recombinant genetic variant G3G3 Homo sapiens
3.1.8.1 0.14
-
paraoxon pH 8.0, recombinant genetic variant G3H9 Homo sapiens
3.1.8.1 0.54
-
paraoxon pH 8.0, recombinant genetic variant G1C4 Homo sapiens
3.1.8.1 0.87
-
paraoxon pH 8.0, recombinant genetic variant G2E6 Homo sapiens
3.1.8.1 0.98
-
paraoxon pH 8.0, recombinant genetic variant G2D6 Homo sapiens
3.1.8.1 1.1
-
paraoxon pH 8.0, recombinant genetic variant G3C9 Homo sapiens
3.1.8.1 1.16
-
paraoxon pH 8.0, recombinant genetic variant G1A5 Homo sapiens
3.1.8.1 1.2
-
paraoxon pH 8.0, recombinant genetic variant G3H8 Homo sapiens
3.1.8.1 3
-
paraoxon pH 8.0, wild-type enzyme Homo sapiens
3.1.8.1 552
-
phenyl acetate pH 8.0, recombinant genetic variant G1C4 Homo sapiens
3.1.8.1 562
-
phenyl acetate pH 8.0, recombinant genetic variant G2D6 Homo sapiens
3.1.8.1 789
-
phenyl acetate pH 8.0, recombinant genetic variant G3C9 Homo sapiens
3.1.8.1 833
-
phenyl acetate pH 8.0, recombinant genetic variant G1A5 Homo sapiens
3.1.8.1 965
-
phenyl acetate pH 8.0, recombinant genetic variant G2E6 Homo sapiens
3.1.8.1 1018
-
phenyl acetate pH 8.0, recombinant genetic variant G3H8 Homo sapiens
3.1.8.1 1236
-
phenyl acetate pH 8.0, recombinant wild-type PON1 Homo sapiens