EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.2 | application of directed evolution in achieving functional expression of PON1 and PON3 in Escherichia coli culture and a dramatic increase in their hydrolytic proficiency toward the fluorogenic organophosphate substrate 7-O-diethylphosphoryl-3-cyano-7-hydroxycoumarin. The powerful tool of directed evolution opens new prospects for improving their phosphotriesterase activity toward other hazardous organophosphates and toward catalytic activities related to the prevention of atherosclerosis | Homo sapiens |
3.1.8.1 | application of directed evolution in achieving functional expression of PON1 and PON3 in Escherichia coli culture and a dramatic increase in their hydrolytic proficiency toward the fluorogenic organophosphate substrate 7-O-diethylphosphoryl-3-cyano-7-hydroxycoumarin. The powerful tool of directed evolution opens new prospects for improving their phosphotriesterase activity toward other hazardous organophosphates and toward catalytic activities related to the prevention of atherosclerosis | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.2 | 0.16 | - |
2-naphthyl acetate | pH 8.0, recombinant genetic variant G1A7 | Homo sapiens | |
3.1.1.2 | 0.18 | - |
2-naphthyl acetate | pH 8.0, recombinant genetic variant G1B11 | Homo sapiens | |
3.1.1.2 | 0.211 | - |
2-naphthyl acetate | pH 8.0 | Oryctolagus cuniculus | |
3.1.1.2 | 0.26 | - |
2-naphthyl acetate | pH 8.0, recombinant genetic variant G2C2 | Homo sapiens | |
3.1.1.2 | 0.5 | - |
2-naphthyl acetate | pH 8.0 | Mus musculus | |
3.1.1.2 | 0.65 | - |
2-naphthyl acetate | pH 8.0, recombinant genetic variant G3G3 | Homo sapiens | |
3.1.8.1 | 0.085 | - |
paraoxon | pH 8.0, recombinant genetic variant G1A5 | Homo sapiens | |
3.1.8.1 | 0.088 | - |
paraoxon | pH 8.0, recombinant genetic variant G3H8 | Homo sapiens | |
3.1.8.1 | 0.089 | - |
paraoxon | pH 8.0, recombinant genetic variant G2E6 | Homo sapiens | |
3.1.8.1 | 0.094 | - |
paraoxon | pH 8.0, recombinant genetic variant G3C9 | Homo sapiens | |
3.1.8.1 | 0.1 | - |
paraoxon | pH 8.0, recombinant genetic variant G2D6 | Homo sapiens | |
3.1.8.1 | 0.12 | - |
paraoxon | pH 8.0, recombinant genetic variant G1C4 | Homo sapiens | |
3.1.8.1 | 0.51 | - |
paraoxon | pH 8.0, recombinant genetic variant G3A5 | Homo sapiens | |
3.1.8.1 | 0.54 | - |
paraoxon | pH 8.0, wild-type enzyme | Homo sapiens | |
3.1.8.1 | 0.75 | - |
paraoxon | pH 8.0, recombinant genetic variant G3G3 | Homo sapiens | |
3.1.8.1 | 0.8 | - |
paraoxon | pH 8.0, recombinant genetic variant G2C2 | Homo sapiens | |
3.1.8.1 | 0.8 | - |
paraoxon | pH 8.0, recombinant genetic variant G3H9 | Homo sapiens | |
3.1.8.1 | 1.1 | - |
paraoxon | pH 8.0, recombinant genetic variant G1B11 | Homo sapiens | |
3.1.8.1 | 1.3 | - |
paraoxon | pH 8.0 | Oryctolagus cuniculus | |
3.1.8.1 | 1.4 | - |
paraoxon | pH 8.0 | Mus musculus | |
3.1.8.1 | 2.5 | - |
paraoxon | pH 8.0, recombinant genetic variant G1A7 | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.2 | Homo sapiens | Q15166 | - |
- |
3.1.1.2 | Mus musculus | Q62087 | - |
- |
3.1.1.2 | Oryctolagus cuniculus | Q9BGN0 | - |
- |
3.1.8.1 | Homo sapiens | P27169 | - |
- |
3.1.8.1 | Homo sapiens | Q15166 | - |
- |
3.1.8.1 | Mus musculus | Q62087 | - |
- |
3.1.8.1 | Oryctolagus cuniculus | Q9BGN0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.2 | - |
Homo sapiens |
3.1.8.1 | - |
Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.2 | 2-naphthyl acetate + H2O | - |
Homo sapiens | 2-naphthol + acetate | - |
? | |
3.1.1.2 | 2-naphthyl acetate + H2O | - |
Oryctolagus cuniculus | 2-naphthol + acetate | - |
? | |
3.1.1.2 | 2-naphthyl acetate + H2O | - |
Mus musculus | 2-naphthol + acetate | - |
? | |
3.1.8.1 | 7-O-diethylphosphoryl-3-cyano-7-hydroxycoumarin + H2O | - |
Homo sapiens | ? | - |
? | |
3.1.8.1 | paraoxon + H2O | - |
Homo sapiens | diethylphosphate + 4-nitrophenol | - |
? | |
3.1.8.1 | paraoxon + H2O | - |
Homo sapiens | 4-nitrophenol + diethyl phosphate | - |
? | |
3.1.8.1 | paraoxon + H2O | - |
Oryctolagus cuniculus | 4-nitrophenol + diethyl phosphate | - |
? | |
3.1.8.1 | paraoxon + H2O | - |
Mus musculus | 4-nitrophenol + diethyl phosphate | - |
? | |
3.1.8.1 | phenyl acetate + H2O | - |
Homo sapiens | phenol + acetate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.8.1 | PON1 | - |
Homo sapiens |
3.1.8.1 | PON3 | - |
Homo sapiens |
3.1.8.1 | PON3 | - |
Oryctolagus cuniculus |
3.1.8.1 | PON3 | - |
Mus musculus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.2 | 0.66 | - |
2-naphthyl acetate | pH 8.0 | Oryctolagus cuniculus | |
3.1.1.2 | 1.9 | - |
2-naphthyl acetate | pH 8.0, recombinant genetic variant G1A7 | Homo sapiens | |
3.1.1.2 | 2 | - |
2-naphthyl acetate | pH 8.0, recombinant genetic variant G1B11 | Homo sapiens | |
3.1.1.2 | 2.12 | - |
2-naphthyl acetate | pH 8.0 | Mus musculus | |
3.1.1.2 | 18.8 | - |
2-naphthyl acetate | pH 8.0, recombinant genetic variant G2C2 | Homo sapiens | |
3.1.1.2 | 26.25 | - |
2-naphthyl acetate | pH 8.0, recombinant genetic variant G3G3 | Homo sapiens | |
3.1.8.1 | 0.0008 | - |
paraoxon | pH 8.0 | Mus musculus | |
3.1.8.1 | 0.001 | - |
paraoxon | pH 8.0 | Oryctolagus cuniculus | |
3.1.8.1 | 0.007 | - |
paraoxon | pH 8.0, recombinant genetic variant G1A7 | Homo sapiens | |
3.1.8.1 | 0.009 | - |
paraoxon | pH 8.0, recombinant genetic variant G1B11 | Homo sapiens | |
3.1.8.1 | 0.036 | - |
paraoxon | pH 8.0, recombinant genetic variant G2C2 | Homo sapiens | |
3.1.8.1 | 0.04 | - |
paraoxon | pH 8.0, recombinant genetic variant G3A5 | Homo sapiens | |
3.1.8.1 | 0.11 | - |
paraoxon | pH 8.0, recombinant genetic variant G3G3 | Homo sapiens | |
3.1.8.1 | 0.14 | - |
paraoxon | pH 8.0, recombinant genetic variant G3H9 | Homo sapiens | |
3.1.8.1 | 0.54 | - |
paraoxon | pH 8.0, recombinant genetic variant G1C4 | Homo sapiens | |
3.1.8.1 | 0.87 | - |
paraoxon | pH 8.0, recombinant genetic variant G2E6 | Homo sapiens | |
3.1.8.1 | 0.98 | - |
paraoxon | pH 8.0, recombinant genetic variant G2D6 | Homo sapiens | |
3.1.8.1 | 1.1 | - |
paraoxon | pH 8.0, recombinant genetic variant G3C9 | Homo sapiens | |
3.1.8.1 | 1.16 | - |
paraoxon | pH 8.0, recombinant genetic variant G1A5 | Homo sapiens | |
3.1.8.1 | 1.2 | - |
paraoxon | pH 8.0, recombinant genetic variant G3H8 | Homo sapiens | |
3.1.8.1 | 3 | - |
paraoxon | pH 8.0, wild-type enzyme | Homo sapiens | |
3.1.8.1 | 552 | - |
phenyl acetate | pH 8.0, recombinant genetic variant G1C4 | Homo sapiens | |
3.1.8.1 | 562 | - |
phenyl acetate | pH 8.0, recombinant genetic variant G2D6 | Homo sapiens | |
3.1.8.1 | 789 | - |
phenyl acetate | pH 8.0, recombinant genetic variant G3C9 | Homo sapiens | |
3.1.8.1 | 833 | - |
phenyl acetate | pH 8.0, recombinant genetic variant G1A5 | Homo sapiens | |
3.1.8.1 | 965 | - |
phenyl acetate | pH 8.0, recombinant genetic variant G2E6 | Homo sapiens | |
3.1.8.1 | 1018 | - |
phenyl acetate | pH 8.0, recombinant genetic variant G3H8 | Homo sapiens | |
3.1.8.1 | 1236 | - |
phenyl acetate | pH 8.0, recombinant wild-type PON1 | Homo sapiens |