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Literature summary extracted from

  • Vickers, T.J.; Greig, N.; Fairlamb, A.H.
    A trypanothione-dependent glyoxalase I with a prokaryotic ancestry in Leishmania major (2004), Proc. Natl. Acad. Sci. USA, 101, 13186-13191.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.5 expression in Escherichia coli Leishmania major

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.5 additional information not inhibitory: S-methyl-glutathione, S-propyl-glutathione, S-butyl-glutathione, S-hexyl-glutathione, S-octyl-glutathione at 1 mM, S-p-nitrobenzyl-glutathione and S-p-bromobenzyl-glutathione at 250 microM Leishmania major

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.5 additional information
-
 S-mandoylglutathione more than 0.08, purified enzyme, pH 7.0, 27°C Leishmania major
4.4.1.5 additional information
-
 (R)-S-lactoylglutathione more than 1.9, purified enzyme, pH 7.0, 27°C Leishmania major
4.4.1.5 0.032
-
 S-D-lactoyltrypanothione purified enzyme, pH 7.0, 27°C Leishmania major
4.4.1.5 0.051
-
 S-mandoyltrypanothione purified enzyme, pH 7.0, 27°C Leishmania major

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.5 Co2+ reactivation of the apoenzyme Leishmania major
4.4.1.5 Mn2+ reactivation of the apoenzyme Leishmania major
4.4.1.5 additional information no activity with Zn2+ Leishmania major
4.4.1.5 Ni2+ reactivation of the apoenzyme, largest recovery in activity Leishmania major

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.5 16607
-
 2 * 16607, mass spectrometry Leishmania major
4.4.1.5 38700
-
 gel filtration Leishmania major

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.5 Leishmania major Q68RJ8 Friedlin A1 clone
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.5
-
 Leishmania major

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.5 additional information
-
 40 nmol/min/mg Leishmania major extract, trypanothione, pH 7.0, 27°C. Below 5 nmol/min/mg Leishmania major extract, pH 7.0, 27°C Leishmania major

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.5 (R)-S-lactoylglutathione
-
 Leishmania major glutathione + methylglyoxal
-
 ?
4.4.1.5 S-D-lactoyltrypanothione
-
 Leishmania major methylglyoxal + reduced trypanothione
-
 ?
4.4.1.5 S-mandoylglutathione
-
 Leishmania major glutathione + phenylglyoxal
-
 ?
4.4.1.5 S-mandoyltrypanothione
-
 Leishmania major phenylglyoxal + trypanothione
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.5 dimer 2 * 16607, mass spectrometry Leishmania major

Synonyms

EC Number Synonyms Comment Organism
4.4.1.5 Glo1
-
 Leishmania major

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.4.1.5 Leishmania major chromatofocusing
-
 4.9