BRENDA - Enzyme Database

Selective inhibition of the citrate-to-isocitrate reaction of cytosolic aconitase by phosphomimetic mutation of serine-711

Pitula, J.S.; Deck, K.M.; Clarke, S.L.; Anderson, S.A.; Vasanthakumar, A.; Eisenstein, R.S.; Proc. Natl. Acad. Sci. USA 101, 10907-10912 (2004)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.3
S711A
citrate-to-isocitrate aconitase activity is about 70% of the wild-type activity, isocitrate-to-cis-aconitate activity is about 90% of wild-type activity
Homo sapiens
4.2.1.3
S711D
no citrate-to-isocitrate aconitase activity, isocitrate-to-cis-aconitate activity is identical to wild-type activity
Homo sapiens
4.2.1.3
S711E
citrate-to-isocitrate aconitase activity is about 10% of the wild-type activity, isocitrate-to-cis-aconitate activity is about 20% of wild-type activity
Homo sapiens
4.2.1.3
S711T
citrate-to-isocitrate aconitase activity is about 60% of the wild-type activity, isocitrate-to-cis-aconitate activity is about 60% of wild-type activity
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.3
cytosol
-
Homo sapiens
5829
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.3
Homo sapiens
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
4.2.1.3
phosphoprotein
S711 of IRP1 is phosphorylated by proteinkinase C in vitro and in vivo in human HEK cells. Aconitase activity of the S711 phosphomimetic mutants is preferentially inhibited in the citrate mode of aconitase function. The substitution of phosphomimetic amino acids for S711 fails to affect the RNA-binding affinity of IRP1
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.3
HEK-293 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
citrate
-
666706
Homo sapiens
isocitrate
-
-
-
r
4.2.1.3
isocitrate
-
666706
Homo sapiens
cis-aconitate + H2O
-
-
-
r
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.3
S711A
citrate-to-isocitrate aconitase activity is about 70% of the wild-type activity, isocitrate-to-cis-aconitate activity is about 90% of wild-type activity
Homo sapiens
4.2.1.3
S711D
no citrate-to-isocitrate aconitase activity, isocitrate-to-cis-aconitate activity is identical to wild-type activity
Homo sapiens
4.2.1.3
S711E
citrate-to-isocitrate aconitase activity is about 10% of the wild-type activity, isocitrate-to-cis-aconitate activity is about 20% of wild-type activity
Homo sapiens
4.2.1.3
S711T
citrate-to-isocitrate aconitase activity is about 60% of the wild-type activity, isocitrate-to-cis-aconitate activity is about 60% of wild-type activity
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.3
cytosol
-
Homo sapiens
5829
-
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
4.2.1.3
phosphoprotein
S711 of IRP1 is phosphorylated by proteinkinase C in vitro and in vivo in human HEK cells. Aconitase activity of the S711 phosphomimetic mutants is preferentially inhibited in the citrate mode of aconitase function. The substitution of phosphomimetic amino acids for S711 fails to affect the RNA-binding affinity of IRP1
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.3
HEK-293 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
citrate
-
666706
Homo sapiens
isocitrate
-
-
-
r
4.2.1.3
isocitrate
-
666706
Homo sapiens
cis-aconitate + H2O
-
-
-
r