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Literature summary extracted from

  • Mehrani, H.
    Simplified procedures for purification and stabilization of human plasma butyrylcholinesterase (2004), Proc. Biochem., 39, 877-882.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.1.1.8 stabilized by glycerol, polythylene glycol, or ammonium sulfate, all best at 20% w/v Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.8 butyrylthiocholine substrate inhibition above 10 mM Homo sapiens
3.1.1.8 EDTA inhibitory above 1 mM Homo sapiens
3.1.1.8 paraoxon 50% inhibition at 0.0028 mM Homo sapiens
3.1.1.8 parathion 50% inhibition at 0.022 mM Homo sapiens
3.1.1.8 propionylthiocholine substrate inhibition above 10 mM Homo sapiens
3.1.1.8 tetra monoisopropyl pyrophosphomide 50% inhibition at 0.055 mM Homo sapiens
3.1.1.8 Zn2+ 1-2 mM, 10% activation, inhibitory above Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.8 0.018
-
 butyrylthiocholine pH 7.4, 30°C Homo sapiens
3.1.1.8 0.083
-
 acetylthiocholine pH 7.4, 30°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.8 Zn2+ 1-2 mM, 10% activation, inhibitory above Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.8 85000
-
 x * 85000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.8 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.8
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.8 blood plasma
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
3.1.1.8 30°C, 20% w/v ammonium sulfate, 12 weeks, 62% loss of activity Homo sapiens
3.1.1.8 30°C, 20% w/v glycerol, 12 weeks, 38% loss of activity Homo sapiens
3.1.1.8 30°C, 20% w/v polyethylene glycol 6000, 12 weeks, 20% loss of activity Homo sapiens
3.1.1.8 4°C, 20% w/v ammonium sulfate, 12 weeks, no loss of activity Homo sapiens
3.1.1.8 4°C, 20% w/v glycerol, 12 weeks, no loss of activity Homo sapiens
3.1.1.8 4°C, 20% w/v polyethylene glycol 6000, 12 weeks, no loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.8 acetylthiocholine + H2O
-
 Homo sapiens acetate + thiocholine
-
 ?
3.1.1.8 butyrylthiocholine + H2O
-
 Homo sapiens thiocholine + butyrate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.8 ? x * 85000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.8 37 42
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.8 7 8
-
 Homo sapiens