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Literature summary extracted from
- Sunflower seed lipase: extraction, purification, and characterization (2005), Prep. Biochem. Biotechnol., 35, 37-51.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.3 | additional information | - |
additional information | kinetics | Helianthus annuus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 22000 | - |
1 * 22000 | Helianthus annuus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.3 | Helianthus annuus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.3 | native enzyme from seed extract supernatant, by salt preciptation fractionation, dialysis of cellulose membrane, and gel filtration | Helianthus annuus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.3 | seed | - |
Helianthus annuus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | additional information | - |
optimal assay time is 5 min | Helianthus annuus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.1.3 | the purified enzyme stable for about 4 months | Helianthus annuus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.3 | triacylglycerol + H2O | the seed enzyme shows a preference for triglyceride with natural monounsaturated fatty acids | Helianthus annuus | diacylglycerol + a carboxylate | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | monomer | 1 * 22000 | Helianthus annuus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | lipase | - |
Helianthus annuus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 50 | - |
- |
Helianthus annuus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 35 | 50 | stable | Helianthus annuus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 7.5 | - |
- |
Helianthus annuus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | additional information | - |
stable at alkaline pH | Helianthus annuus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.1.3 | Helianthus annuus | - |
- |
8 |
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