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Literature summary extracted from

  • Klaus, D.; Ohlrogge, J.B.; Neuhaus, H.E.; Dormann, P.
    Increased fatty acid production in potato by engineering of acetyl-CoA carboxylase (2004), Planta, 219, 389-396.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.4.1.2 overexpression of isozyme ACC1 in the amyloplast of Solanum tuberosum tubers using the Agrobacterium transfection system Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
6.4.1.2 additional information construction of transgenic potato plants by overexpression of the Arabidopsis thaliana isozyme ACC1 in the amyloplast of Solanum tuberosum tubers leads to an increased fatty acid synthesis and a 5fold increased amount of triacylglycerol in the tubers Arabidopsis thaliana
6.4.1.2 additional information construction of transgenic potato plants by overexpression of the Arabidopsis thaliana isozyme ACC1 in the amyloplast of tubers leads to an increased fatty acid synthesis and a 5fold increased amount of triacylglycerol in the tubers, up to 10fold increased enzyme activity in transgenic plants Solanum tuberosum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.2 Mg2+
-
 Solanum tuberosum
6.4.1.2 Mg2+
-
 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.2 200000
-
 x * 200000, SDS-PAGE Solanum tuberosum
6.4.1.2 200000
-
 x * 200000, recombinant enzyme, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3- Arabidopsis thaliana
-
 ADP + malonyl-CoA + phosphate
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3- Solanum tuberosum the enzyme activity is limiting for lipid synthesis in tubers, biosynthetic pathway and regulatory aspects, overview ADP + malonyl-CoA + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.2 Arabidopsis thaliana
-
 isozyme ACC1
-
6.4.1.2 Solanum tuberosum
-
 cv. Desiree
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.4.1.2 seed
-
 Arabidopsis thaliana
-
6.4.1.2 tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.2 0.000009
-
 wild-type tuber Solanum tuberosum
6.4.1.2 0.000086 0.000096 transgenic tubers highly overexpressing the Arabidopsis thaliana enzyme Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Solanum tuberosum ADP + malonyl-CoA + phosphate
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Arabidopsis thaliana ADP + malonyl-CoA + phosphate
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3- the enzyme activity is limiting for lipid synthesis in tubers, biosynthetic pathway and regulatory aspects, overview Solanum tuberosum ADP + malonyl-CoA + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.2 ? x * 200000, SDS-PAGE Solanum tuberosum
6.4.1.2 ? x * 200000, recombinant enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
6.4.1.2 ACC1
-
 Solanum tuberosum
6.4.1.2 ACC1
-
 Arabidopsis thaliana
6.4.1.2 ACCase
-
 Solanum tuberosum
6.4.1.2 ACCase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.4.1.2 30
-
 assay at Solanum tuberosum
6.4.1.2 30
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.2 8.2
-
 assay at Solanum tuberosum
6.4.1.2 8.2
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.2 ATP
-
 Solanum tuberosum
6.4.1.2 ATP
-
 Arabidopsis thaliana