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Literature summary extracted from

  • Andriotis, V.M.; Ross, J.D.
    Isolation and partial characterisation of acid phosphatase isozymes from dormant oilseed of Corylus avellana L (2004), Planta, 219, 346-358.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 fluoride
-
Corylus avellana
3.1.3.2 phosphate competitive product inhibition Corylus avellana
3.1.3.2 vanadate
-
Corylus avellana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 additional information
-
additional information steady-state kinetics of the 3 isozymes Corylus avellana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 81500
-
gel filtration Corylus avellana

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Corylus avellana
-
several isozymes
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.2 glycoprotein all 3 isozymes from seeds Corylus avellana

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 3 isozymes 75fold, 389fold, and 191fold from seed cotyledons to homogeneity by successive ammonoum sulfate fractionation, gel filtration, concanavalin A affinity chromatography, and cation- and anion exchange chromatography Corylus avellana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 cotyledon
-
Corylus avellana
-
3.1.3.2 seed dormant, hazelnut, several isozymes Corylus avellana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 45.8
-
purified APase1 Corylus avellana
3.1.3.2 117
-
purified APase3 Corylus avellana
3.1.3.2 239
-
purified APase2 Corylus avellana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 ADP + H2O substrate only of isozyme APase2 Corylus avellana AMP + phosphate
-
?
3.1.3.2 ATP + H2O
-
Corylus avellana ADP + phosphate
-
?
3.1.3.2 D-fructose 6-phosphate + H2O
-
Corylus avellana D-fructose + phosphate
-
?
3.1.3.2 D-glucose 6-phosphate + H2O
-
Corylus avellana D-glucose + phosphate
-
?
3.1.3.2 diphosphate + H2O
-
Corylus avellana 2 phosphate
-
?
3.1.3.2 glycerophosphate + H2O
-
Corylus avellana glycerol + phosphate
-
?
3.1.3.2 additional information substrate specificity of isozymes APase1-3, no activity with AMP and phytic acid, all 3 isozymes do not show phytase activity, while APase 1 and 2 show significant phosphotyrosine phosphatase activity Corylus avellana ?
-
?
3.1.3.2 O-phospho-L-serine + H2O
-
Corylus avellana L-serine + phosphate
-
?
3.1.3.2 O-phospho-L-threonine + H2O
-
Corylus avellana L-threonine + phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 APase
-
Corylus avellana
3.1.3.2 orthophosphoric-monoester phosphohydrolase
-
Corylus avellana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 3
-
APase1 Corylus avellana
3.1.3.2 5.6
-
APase2 Corylus avellana
3.1.3.2 6
-
APase3 Corylus avellana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.2 Corylus avellana APase1
-
5.1
3.1.3.2 Corylus avellana APase2
-
6.9
3.1.3.2 Corylus avellana APase3
-
7.3