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Literature summary extracted from

  • Jones, A.L.; Gane, A.M.; Herbert, D.; Willey, D.L.; Rutter, A.J.; Kille, P.; Dancer, J.E.; Harwood, J.L.
    beta-Ketoacyl-acyl carrier protein synthase III from pea (Pisum sativum L.): properties, inhibition by a novel thiolactomycin analogue and isolation of a cDNA clone encoding the enzyme (2003), Planta, 216, 752-761.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.180 DNA and amino acid sequence determination and analysis, phylogenetic tree, expression as GST-fusion enzyme in Escherichia coli Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.180 thiolactomycin uncompetitive, IC50 is 0.7 mM Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.180 0.009
-
 malonyl-[acyl-carrier protein] pH 7.6, 30°C Pisum sativum
2.3.1.180 0.016
-
 acetyl-CoA pH 7.6, 30°C Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.180 42000
-
 2 * 42000, about, DNA sequence calculation Pisum sativum
2.3.1.180 63000 75000 gel filtration Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] Pisum sativum
-
 acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.180 Pisum sativum Q9FSD6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.180 native enzyme 147fold from leaves by ammonium sulfate fractionation, ion exchange chromatography, hydrophobic interaction chromatography on a butyl resin, and affinity chromatography, recombinant GST-fusion enzyme from Escherichia coli by glutathione affinity chromatography, GST is cleaved off by thrombin Pisum sativum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.180 acetyl-CoA + a malonyl-[acyl-carrier protein] = an acetoacetyl-[acyl-carrier protein] + CoA + CO2 catalytic residues are Cys123, His323, and Asn353 Pisum sativum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.180 leaf
-
 Pisum sativum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Pisum sativum acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 additional information the enzyme shows also acetyl-CoA-[acyl-carrier-protein] transacylase activity Pisum sativum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.180 dimer 2 * 42000, about, DNA sequence calculation Pisum sativum

Synonyms

EC Number Synonyms Comment Organism
2.3.1.180 ACP synthase III
-
 Pisum sativum
2.3.1.180 beta-ketoacyl-acyl carrier protein synthase III
-
 Pisum sativum
2.3.1.180 KAS III
-
 Pisum sativum
2.3.1.180 short-chain condensing enzyme
-
 Pisum sativum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.180 30
-
 assay at Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.180 7.6
-
-
 Pisum sativum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.180 0.7
-
 uncompetitive, IC50 is 0.7 mM Pisum sativum thiolactomycin