EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.28 | EDTA | inhibits the acid trehalase by 70% | Phaseolus vulgaris | |
3.2.1.28 | Fe3+ | inhibits the acid trehalase by 50% | Phaseolus vulgaris | |
3.2.1.28 | Hg+ | inhibits the acid trehalase by 95% | Phaseolus vulgaris | |
3.2.1.28 | validamycin A | inhibits the acid trehalase | Phaseolus vulgaris |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.28 | 0.109 | - |
alpha,alpha-trehalose | pH 6.3, 37°C | Phaseolus vulgaris |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.28 | Co2+ | activates the acid trehalase | Phaseolus vulgaris | |
3.2.1.28 | K+ | activates the acid trehalase | Phaseolus vulgaris | |
3.2.1.28 | Li+ | activates the acid trehalase | Phaseolus vulgaris | |
3.2.1.28 | Mg2+ | activates the acid trehalase by about 30% | Phaseolus vulgaris | |
3.2.1.28 | Mn2+ | activates the acid trehalase by about 30% | Phaseolus vulgaris | |
3.2.1.28 | Na+ | activates the acid trehalase by about 30% | Phaseolus vulgaris |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 42000 | - |
x * 42000 + x * 45000, SDS-PAGE | Phaseolus vulgaris |
3.2.1.28 | 45000 | - |
x * 42000 + x * 45000, SDS-PAGE | Phaseolus vulgaris |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.28 | alpha,alpha-trehalose + H2O | Phaseolus vulgaris | - |
2 D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.28 | Phaseolus vulgaris | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.28 | acid trehalase 425fold from root nodules, by ammonium sulfate fractionation, ion exchange chromatography, gel filtration, and native PAGE | Phaseolus vulgaris |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.28 | root nodule | - |
Phaseolus vulgaris | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 175.7 | - |
purified acid trehalase | Phaseolus vulgaris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.28 | alpha,alpha-trehalose + H2O | - |
Phaseolus vulgaris | 2 D-glucose | - |
? | |
3.2.1.28 | alpha,alpha-trehalose + H2O | preferred substrate of acid trehalase | Phaseolus vulgaris | 2 D-glucose | - |
? | |
3.2.1.28 | cellobiose + H2O | - |
Phaseolus vulgaris | 2 D-glucose | - |
? | |
3.2.1.28 | maltose + H2O | - |
Phaseolus vulgaris | 2 D-glucose | - |
? | |
3.2.1.28 | melibiose + H2O | - |
Phaseolus vulgaris | D-glucose + D-galactose | - |
? | |
3.2.1.28 | additional information | no activity of acid trehalase with lactose and turanose | Phaseolus vulgaris | ? | - |
? | |
3.2.1.28 | raffinose + H2O | - |
Phaseolus vulgaris | ? | - |
? | |
3.2.1.28 | sucrose + H2O | - |
Phaseolus vulgaris | D-glucose + D-fructose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.28 | ? | x * 42000 + x * 45000, SDS-PAGE | Phaseolus vulgaris |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.28 | acid trehalase | - |
Phaseolus vulgaris |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 37 | - |
assay at | Phaseolus vulgaris |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 25 | 75 | - |
Phaseolus vulgaris |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 40 | - |
30 min, stable up to | Phaseolus vulgaris |
3.2.1.28 | 45 | 50 | loss of 40% activity, purified acid trehalase | Phaseolus vulgaris |
3.2.1.28 | 60 | - |
nearly complete inactivation of purified acid trehalase | Phaseolus vulgaris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 3.9 | - |
acid trehalase | Phaseolus vulgaris |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 3.6 | 6.6 | - |
Phaseolus vulgaris |